CAJ | 학술논문

采用ISSR分子标记技术对扁蓿豆4个品系的遗传多样性、遗传差异和遗传结构进行了研究.用7个有效引物对4个品系进行PCR扩增,共获得73个等位基因位点,每条引物平均检测出10.4个位点,其中多态性位点69个,多态位点百分率为94.5%.各品系的 Nei's遗传多样性为0.2569,Shannon's信息指数为0.4046.Nei's指数估算和分子方差分析均表明,扁蓿豆4个品系的遗传多样性主要存在于品系内,4个品系间亦出现了较大的遗传分化(G_(st)=0.1324).品系00-61、90-36、00-81和93-21的多态位点百分率分别为73.97%、79.45%、 82.19%和83.56%;Nei's遗传多样性(H)分别为0.2050、0.2153、0.2264和0.2474;Shannon's信息指数(I)分别为0.3222、0.3396、0.3538和0.3821.各品系的遗传参数大小排序均为品系93-21＞品系00-81＞品系90-36＞品系00-61.采用系统聚类和主坐标分析(PCA)两种方法所获得的聚类结果一致,即品系00-61和00-81遗传差异相对较小,其次是品系90-36,品系93-21与各品系的遗传差异较大.
채용ISSR분자표기기술대편숙두4개품계적유전다양성、유전차이화유전결구진행료연구.용7개유효인물대4개품계진행PCR확증,공획득73개등위기인위점,매조인물평균검측출10.4개위점,기중다태성위점69개,다태위점백분솔위94.5%.각품계적 Nei's유전다양성위0.2569,Shannon's신식지수위0.4046.Nei's지수고산화분자방차분석균표명,편숙두4개품계적유전다양성주요존재우품계내,4개품계간역출현료교대적유전분화(G_(st)=0.1324).품계00-61、90-36、00-81화93-21적다태위점백분솔분별위73.97%、79.45%、 82.19%화83.56%;Nei's유전다양성(H)분별위0.2050、0.2153、0.2264화0.2474;Shannon's신식지수(I)분별위0.3222、0.3396、0.3538화0.3821.각품계적유전삼수대소배서균위품계93-21＞품계00-81＞품계90-36＞품계00-61.채용계통취류화주좌표분석(PCA)량충방법소획득적취류결과일치,즉품계00-61화00-81유전차이상대교소,기차시품계90-36,품계93-21여각품계적유전차이교대.
The genetic diversity, genetic difference and genetic structure of four strains of Melilotoides ruthenica were investigated with ISSR marker. Seven ISSR primers generated highly reproducible and stable DNA fragments. 69 of 73 loci were polymorphic (PPB=94.5%). The results of Popgen32 analysis indicated that Nei's gene diversity(H) was 0.2569, Shannon information index(I) was 0.4046. Nei's analysis and AMOVA analysis both indicated that the majority genetic variation of M. ruthenica strains occurred within strains, higher variations also occurred among the strains (G_(st)=0.1324). The PPB of strains 00-61,90-36,00-81 and 93-21 was 73.97%, 79.45%, 82.19% and 83.56%, respectively;Nei's gene diversity(H) was 0.2050, 0.2153, 0.2264 and 0.2474, respectively;Shannon information index(I) was 0.3222, 0.3396, 0.3538 and 0.3821, respectively. The sequence of all genetic index above was 93-21＞00-81＞90-36＞00-61.The results of cluster analysis and principal coordinate analysis (PCA) were similar. The genetic difference between strain 00-61 and 00-81 was relatively lower, second was strain 90-36, and strain 93-21 had higher genetic difference from other strains.