CAJ | 학술논문

为研究猪卵母细胞发生早期卵巢上细胞的凋亡情况,制作妊娠33、40、46、54、61 d母猪胎儿卵巢的石蜡切片,并进行荧光染色.结果发现,母猪妊娠到33 d时,胎猪卵巢上即可见原始生殖细胞和卵原细胞的凋亡,随着妊娠时间的延长,凋亡也逐渐增加,妊娠33、40、46、54和61 d母猪胎儿卵巢上卵原细胞的凋亡率分别为3.13%、4.32%、 5.50%、9.43%和11.10%,同时,颗粒细胞的凋亡率则分别为8.89%、12.45%、14.74%、17.96%和22.81%.结果说明,在猪胎儿卵母细胞发生早期,即开始发生了卵原细胞和颗粒细胞的凋亡,且有逐渐增加的趋势.
위연구저란모세포발생조기란소상세포적조망정황,제작임신33、40、46、54、61 d모저태인란소적석사절편,병진행형광염색.결과발현,모저임신도33 d시,태저란소상즉가견원시생식세포화란원세포적조망,수착임신시간적연장,조망야축점증가,임신33、40、46、54화61 d모저태인란소상란원세포적조망솔분별위3.13%、4.32%、 5.50%、9.43%화11.10%,동시,과립세포적조망솔칙분별위8.89%、12.45%、14.74%、17.96%화22.81%.결과설명,재저태인란모세포발생조기,즉개시발생료란원세포화과립세포적조망,차유축점증가적추세.
Oogenesis is an important physiological event in the process of reproductive development.The present study was undertaken to investigate the apoptosis of the cells on fetal porcine ovaries.The fetal porcine ovaries 33,40,46,54 and 61 days post coitum (dpc) were collected from pregnant sows,respectively,and the paraffin sections were prepared and then stained by fluorescent dye Hoechst 33258.The results showed that the apoptotic cells were observed since 33 dpc in primordial germ cells and oogonium,and the apoptosis rates were 3.13%,4.32%,5.50%,9.43% and 11.10% at 33,40,46,54 and 61 dpc respectively.Meanwhile,the apoptosis rates of granulosa cells were 8.89%,12.45%,14.74%,17.96% and 22.81% at 33,40,46,54 and 61 dpc respectively.These results suggest that the apoptosis of oogonium and follicle cells on fetal porince ovaries happens and increases at the early stage of oogenesis.