中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
3期
295-298
,共4页
枸杞多糖%锰中毒%学习记忆%神经发生%5-溴脱氧尿嘧啶核苷
枸杞多糖%錳中毒%學習記憶%神經髮生%5-溴脫氧尿嘧啶覈苷
구기다당%맹중독%학습기억%신경발생%5-추탈양뇨밀정핵감
Lycium barbarum polysaccharides%manganese poisoning%learning & memory%neurogenesis%bromodeoxyuridine
目的 探讨枸杞多糖(Lyeium barbarum polysaccharides,LBP)对锰中毒小鼠神经发生和学习记忆的影响.方法 选择健康成年昆明小鼠随机分5组,分别为对照组,锰中毒组,锰中毒加LBP高、中、低剂量组.LBP和氯化锰分剐采用灌胃和腹腔注射方法 给药.用Morris水迷宫训练测试其空间学习争记忆能力;用5-溴脱氧尿嘧啶核苷(BrdU)标记神经发生细胞.结果锰中毒组平均逃避潜伏期高于对照组(P＜0.05),穿越平台区次数明显低于对照组(P＜0.05);锰中毒加LBP各剂量组海马齿状回(DG)内BrdU阳性细胞数目显著高于锰中毒组(P＜0.05).结论 LBP可能通过促进海马神经发生,从而影响小鼠的学习记忆能力.
目的 探討枸杞多糖(Lyeium barbarum polysaccharides,LBP)對錳中毒小鼠神經髮生和學習記憶的影響.方法 選擇健康成年昆明小鼠隨機分5組,分彆為對照組,錳中毒組,錳中毒加LBP高、中、低劑量組.LBP和氯化錳分剮採用灌胃和腹腔註射方法 給藥.用Morris水迷宮訓練測試其空間學習爭記憶能力;用5-溴脫氧尿嘧啶覈苷(BrdU)標記神經髮生細胞.結果錳中毒組平均逃避潛伏期高于對照組(P＜0.05),穿越平檯區次數明顯低于對照組(P＜0.05);錳中毒加LBP各劑量組海馬齒狀迴(DG)內BrdU暘性細胞數目顯著高于錳中毒組(P＜0.05).結論 LBP可能通過促進海馬神經髮生,從而影響小鼠的學習記憶能力.
목적 탐토구기다당(Lyeium barbarum polysaccharides,LBP)대맹중독소서신경발생화학습기억적영향.방법 선택건강성년곤명소서수궤분5조,분별위대조조,맹중독조,맹중독가LBP고、중、저제량조.LBP화록화맹분과채용관위화복강주사방법 급약.용Morris수미궁훈련측시기공간학습쟁기억능력;용5-추탈양뇨밀정핵감(BrdU)표기신경발생세포.결과맹중독조평균도피잠복기고우대조조(P＜0.05),천월평태구차수명현저우대조조(P＜0.05);맹중독가LBP각제량조해마치상회(DG)내BrdU양성세포수목현저고우맹중독조(P＜0.05).결론 LBP가능통과촉진해마신경발생,종이영향소서적학습기억능력.
Objective To study the effect of Lycium barbarum polysaccharides (LBP) on neurogenesis and learning & memory of manganese poisoning mice.Methods Healthy adult Kunming mice were divided into 5 groups,the control group (A),the manganese poisoning (by manganese chloride peritoneal injection) group (B),the manganese poisoning and treated with gastric perfusion of high,medium,low dosage LBP groups (C,D and E).The spatial learning & memory capacity of mouse was determined by Morris water maze training test.The neurogenetic cells were labelled with bromodeoxyuridine (BrdU) and detected by immunohistochemistry.Results The average escape latency was significantly higher and the times of passing through platform lower in group B than those in group A(P＜0.05).BrdU positive cells in groups C,D and E were significantly more than those in group B (P＜0.05).Conclusion LBP could enhance the learning & memory capability of the manganese poisoning mice by promoting neurogenesis in hippocampus.
