CAJ | 학술논문

目的检测乙型肝炎患者骨髓造血干细胞(HSC)诱导的树突状细胞(DC)的表面分子的表达,评价其相关因素及临床意义.方法收集慢性乙型肝炎患者的骨髓液9例,健康者7例后用磁珠分离仪分离纯化骨髓液CD34+细胞,在含有干细胞生长因子(SCF)、酪氨酸激酶受体家族Ⅲ的配体(FLT3)、促血小板生成素(TPO)、IL-3和10%FBS的IMDM培养基中孵育并进行扩增,在干细胞扩增基础上,在GM-CSF和IL-4作用下诱生DC,通过流式细胞仪分析其表面分子的表达并对DC进行形态学观察.结果乙型肝炎患者HSC经诱导为DC后,其免疫表型CD80、CD86、CD1a的表达均低于正常对照组,差异有统计学意义:CD1a(t=3.94,P＜0.05),CD80(t=7.08,P＜0.01),CD86(t=3.65,P＜0.05),而HLA-DR表达与正常组比较差异无统计学意义(t=0.34,P＞0.05).结论慢性乙型肝炎患者HSC来源的部分DC的表面分子表达低下可能与HBV感染HSC后出现病态的免疫细胞分化有关.
목적 검측을형간염환자골수조혈간세포(HSC)유도적수돌상세포(DC)적표면분자적표체,평개기상관인소급림상의의.방법 수집만성을형간염환자적골수액9례,건강자7례후용자주분리의분리순화골수액CD34+세포,재함유간세포생장인자(SCF)、락안산격매수체가족Ⅲ적배체(FLT3)、촉혈소판생성소(TPO)、IL-3화10%FBS적IMDM배양기중부육병진행확증,재간세포확증기출상,재GM-CSF화IL-4작용하유생DC,통과류식세포의분석기표면분자적표체병대DC진행형태학관찰.결과 을형간염환자HSC경유도위DC후,기면역표형CD80、CD86、CD1a적표체균저우정상대조조,차이유통계학의의:CD1a(t=3.94,P＜0.05),CD80(t=7.08,P＜0.01),CD86(t=3.65,P＜0.05),이HLA-DR표체여정상조비교차이무통계학의의(t=0.34,P＞0.05).결론 만성을형간염환자HSC래원적부분DC적표면분자표체저하가능여HBV감염HSC후출현병태적면역세포분화유관.
Objective To determine the expression of surface molecules of dentritic cells (DCs) derived from marrow haematopoietic stem cell (HSC) of patients with hepatitis B and its clinical significance.Methods The marrows were collected from 9 patients with hepatitis B and 7 healthy donors. The CD34 + cells were isolated and purified by mini-MACS ,then seeded in 12-well plates and cultured in IMDM complete culture medium with 10% FCS and cytokines (SCF,FL3, TPO, IL-3 ). Following the proliferation, DCs were induced by adding GM-CSF,IL-4,TNF for extra 7 days, and the expression of surface molecules on DCs was examined by flow cytometry and the morpholog of stem cells and DCs were observed by microscopy. Results The expression of CD80, CD86, CD1a on DCs derived from haematopoietic stem cells of patients with hepatitis B was in low level compared with that of DCs from healthy donor: CD1a: t = 3.94, P< 0.05; CD80: t = 7. 08, P < 0. 01;CD86:t = 3. 65 ,P <0.05. But the expression of HLA-DR in both gnoups was not significant different ( t =0. 34,P > 0. 05 ) Conclusion The expression of surface molecules on DCs derived from marrow haematopoietic stem cell of patients with hepatitis B decreaded compared with healthy control, which may be related to unhealthy dif ferentiation of haematopoietic stem cells with infection of HBV.