中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
9期
729-731
,共3页
巩方明%刘洪俊%石玉龙%李辰生
鞏方明%劉洪俊%石玉龍%李辰生
공방명%류홍준%석옥룡%리신생
胃肿瘤%DNA甲基化%病理学,临床
胃腫瘤%DNA甲基化%病理學,臨床
위종류%DNA갑기화%병이학,림상
Stomach neoplasms%DNA Methylation%Pathology,clinical
目的 检测胃癌组织中p27基因的5'CpG岛甲基化状态，并探讨p27基因甲基化状态与胃癌生物学特性的关系。方法 利用甲基化特异性PCR测定49例胃癌组织，20例癌旁组织以及10例正常胃黏膜组织中p27基因的甲基化状态。结果 胃癌组p27基因甲基化率为49％，癌旁组p27基因甲基化率为25％，正常胃黏膜组p27基因甲基化率为0。胃癌组p27基因甲基化率明显高于正常胃黏膜组(P＜0.05)；淋巴结转移组p27基因甲基化率为61％，显著高于无淋巴转移组的28％(P＜0.05)。P27基因甲基化率与患者年龄、性别、肿瘤组织大小、部位无关(P＞0.05)。结论 p27基因甲基化可能导致p27mRNA的失转录，并与胃癌的发生、转移相关。
目的 檢測胃癌組織中p27基因的5'CpG島甲基化狀態，併探討p27基因甲基化狀態與胃癌生物學特性的關繫。方法 利用甲基化特異性PCR測定49例胃癌組織，20例癌徬組織以及10例正常胃黏膜組織中p27基因的甲基化狀態。結果 胃癌組p27基因甲基化率為49％，癌徬組p27基因甲基化率為25％，正常胃黏膜組p27基因甲基化率為0。胃癌組p27基因甲基化率明顯高于正常胃黏膜組(P＜0.05)；淋巴結轉移組p27基因甲基化率為61％，顯著高于無淋巴轉移組的28％(P＜0.05)。P27基因甲基化率與患者年齡、性彆、腫瘤組織大小、部位無關(P＞0.05)。結論 p27基因甲基化可能導緻p27mRNA的失轉錄，併與胃癌的髮生、轉移相關。
목적 검측위암조직중p27기인적5'CpG도갑기화상태，병탐토p27기인갑기화상태여위암생물학특성적관계。방법 이용갑기화특이성PCR측정49례위암조직，20례암방조직이급10례정상위점막조직중p27기인적갑기화상태。결과 위암조p27기인갑기화솔위49％，암방조p27기인갑기화솔위25％，정상위점막조p27기인갑기화솔위0。위암조p27기인갑기화솔명현고우정상위점막조(P＜0.05)；림파결전이조p27기인갑기화솔위61％，현저고우무림파전이조적28％(P＜0.05)。P27기인갑기화솔여환자년령、성별、종류조직대소、부위무관(P＞0.05)。결론 p27기인갑기화가능도치p27mRNA적실전록，병여위암적발생、전이상관。
Objective To detect methylation status of the 5'CpG island locating in the promoter region of p27 gene in gastric carcinoma and to analyze the relationship between the aberrant methylaion of p27 gene and the bionomics of gastric carcinoma. Methods Methylation-specific PCR were used to detect the methylation status of p27 in gastric carcinoma( n =49), adjacent tissue of carcinoma ( n =20) and normal tissue(n =10), respectively.Results The mathylation rate is 49％ (24/49) for gastria cancer, 25％(5/20) for tumor-sideward mucosa and 0 (0/10)for normal mucosa. There was a significant positive correlation between gastric carcinoma specimens and normal mucosa specimens ( P ＜ 0. 05 ). There was no significant diference between normal gastric group and tumor-sideward mucosa group ( P ＞ 0. 05 ) and no significant diference between tumor-sideward mucosa group and tumor group ( P ＞ 0. 05 ). The mathylation rate of p27 gene with lymph node metastases (61％) are higher than those without metastasis (28％) (P ＜ 0. 05). And there is no relation on patients' age, sex, tumor size and location.Conclusion p27 gene methylation of the p27 gene is correlated to oncogenesis and metastasis of gastric carcinoma and could lead to the loss of p27mRNA transcription.
