中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
3期
173-175
,共3页
何雅青%张宏斌%姚相杰%廖玉学%杨洪%冼慧霞%阳帆%张海龙%杨小柯%许文波
何雅青%張宏斌%姚相傑%廖玉學%楊洪%冼慧霞%暘帆%張海龍%楊小柯%許文波
하아청%장굉빈%요상걸%료옥학%양홍%승혜하%양범%장해룡%양소가%허문파
肠道病毒属%种系发生%基因
腸道病毒屬%種繫髮生%基因
장도병독속%충계발생%기인
Enterovirus%Phytogeny%Genes
目的 研究深圳地区肠道病毒71型VP1基因变异趋势.方法 用肠道病毒71型特异性引物进行RT-PCR,并对EV71的VP1进行扩增,所得的序列与肠道病毒71型A、B、C基因型代表株的核苷酸序列比较,用DNA Star、BioEdit和Mega 3.1软件进行系统进化分析.结果 35株病毒间VP1核苷酸同源性为92.1%-100%,它们与A、B基因型代表株VP1区核苷酸同源性差异较大,为81.4%-91.1%,与C4基因型代表株接近,其核苷酸同源性在93%-97.4%之间.1998-2004年深圳地区EV71主要流行株为C4b亚型,从2003年开始逐步向C4a亚型过渡,至2006年已全部变迁为C4a亚型,且从2003年至2008年,深圳地区EV71 C4a株与安徽阜阳株FY23的核苷酸的同源性分别为:94.5%-94.7%,95.7%-95.8%,96.2%,95.4%-97.5%,96.3%-99.2%,有逐年增高的趋势.2006年两株EV71和2008年EV71代表株核苷酸序列在VP1区的第66位点,均由A→C,从而导致VP1区氨基酸的第22位点由谷酰胺变为组氨酸(Q→H).结论 深圳地区EV71流行株逐渐由C4b亚型向C4a亚型演变,2006年部分EV71及2008年EV71 VP1第66核苷酸位点发生有义突变.
目的 研究深圳地區腸道病毒71型VP1基因變異趨勢.方法 用腸道病毒71型特異性引物進行RT-PCR,併對EV71的VP1進行擴增,所得的序列與腸道病毒71型A、B、C基因型代錶株的覈苷痠序列比較,用DNA Star、BioEdit和Mega 3.1軟件進行繫統進化分析.結果 35株病毒間VP1覈苷痠同源性為92.1%-100%,它們與A、B基因型代錶株VP1區覈苷痠同源性差異較大,為81.4%-91.1%,與C4基因型代錶株接近,其覈苷痠同源性在93%-97.4%之間.1998-2004年深圳地區EV71主要流行株為C4b亞型,從2003年開始逐步嚮C4a亞型過渡,至2006年已全部變遷為C4a亞型,且從2003年至2008年,深圳地區EV71 C4a株與安徽阜暘株FY23的覈苷痠的同源性分彆為:94.5%-94.7%,95.7%-95.8%,96.2%,95.4%-97.5%,96.3%-99.2%,有逐年增高的趨勢.2006年兩株EV71和2008年EV71代錶株覈苷痠序列在VP1區的第66位點,均由A→C,從而導緻VP1區氨基痠的第22位點由穀酰胺變為組氨痠(Q→H).結論 深圳地區EV71流行株逐漸由C4b亞型嚮C4a亞型縯變,2006年部分EV71及2008年EV71 VP1第66覈苷痠位點髮生有義突變.
목적 연구심수지구장도병독71형VP1기인변이추세.방법 용장도병독71형특이성인물진행RT-PCR,병대EV71적VP1진행확증,소득적서렬여장도병독71형A、B、C기인형대표주적핵감산서렬비교,용DNA Star、BioEdit화Mega 3.1연건진행계통진화분석.결과 35주병독간VP1핵감산동원성위92.1%-100%,타문여A、B기인형대표주VP1구핵감산동원성차이교대,위81.4%-91.1%,여C4기인형대표주접근,기핵감산동원성재93%-97.4%지간.1998-2004년심수지구EV71주요류행주위C4b아형,종2003년개시축보향C4a아형과도,지2006년이전부변천위C4a아형,차종2003년지2008년,심수지구EV71 C4a주여안휘부양주FY23적핵감산적동원성분별위:94.5%-94.7%,95.7%-95.8%,96.2%,95.4%-97.5%,96.3%-99.2%,유축년증고적추세.2006년량주EV71화2008년EV71대표주핵감산서렬재VP1구적제66위점,균유A→C,종이도치VP1구안기산적제22위점유곡선알변위조안산(Q→H).결론 심수지구EV71류행주축점유C4b아형향C4a아형연변,2006년부분EV71급2008년EV71 VP1제66핵감산위점발생유의돌변.
Objective Genetic evolution of VP1 of enterovirus type 71 in Shenzhen were analyzed. Methods All samples were tested by RT-PCR using EV71 specific primer. The VP1 of EV71 were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with subgenotype A, B and C using DNAStar,BioEdit and Mega 3. 1 software. Results Among 35 strains, the homogeneity of the VP1 nucleotide sequence was between 92. 1%-100%. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was between 81. 4% -91. 1%. The VP1 nucleotide sequence of 35 strains of Shenzhen shared between 93% -97. 4% identity with cluster C4. The prevalence strains of EV71 were cluster C4b from 1998 to 2004, and gradually moved to C4a since 2003. All of EV71 were C4b from 2006 to 2008. Also, the homogeneity of the VP1 nucleotide sequence with Anhui FY23 EV71 strain were 94.5% -94. 7% , 95. 7% -95. 8% , 96. 2% , 95.4% -97. 5% , 96. 3% -99.2% from 2003 to 2008. It shows that the homogeneity was increased year by year. There was a mutation ( A→C) at No. 66 nucleotide of VP1 of EV71 that two strains were isolated in 2003 and 8 strains in 2008, that caused amino acid mutation ( Q→H) at No. 22 of VP1. Conclusion EV71 C4b was gradually moved to C4a from 1998 to 2008. There was a missense mutation at No. 66 nucleotide of VP1.
