CAJ | 학술논문

目的研究系统性红斑狼疮(SLE)患者外周血CD4+ CD39+ T细胞中FOXP3蛋白的表达情况,以及糖皮质激素治疗的影响.方法采用流式细胞术检测47例SLE患者(其中29例为初发未经治疗的活动期SLE)和22名正常人外周血CD4+ CD25+ CD39+ T细胞、CD4+CD25+ FOXP3+ T细胞及CD4+ CD39+ FOXP3+ T细胞百分率以及FOXP3蛋白的表达,分析3组细胞之间的相关性及糖皮质激素治疗的影响.结果 SLE活动组、缓解组、正常对照组外周血CD4+ CD25+ CD39+ T细胞百分率分别为(1.3±0.5)%、(1.9±0.8)%、(2.3±1.0)%,该群细胞在SLE活动组中的表达水平低于缓解组和正常对照组(均P<0.05),而在后2组之间差异无统计学意义(P>0.05);SLE活动组中CD4+ CD25+、CD4+ CD25high及CD4+ CD39+ T细胞表达的FOXP3蛋白百分率分别为(45±12)%、(65±14)%、(70±14)%,FOXP3蛋白在CD4+ CD39+ T细胞和CD4+ CD25highT细胞中的表达水平明显高于在CD4+CD25+T细胞中的表达水平(P<0.01),而在CD4+ CD39+T细胞与CD4+CD25highT细胞中的表达水平差异均无统计学意义(均P>0.05).结论 CD39可能是调节性T细胞较好的表面标记,CD39+ Treg细胞表达异常可能参与SLE的发病机制.
목적 연구계통성홍반랑창(SLE)환자외주혈CD4+ CD39+ T세포중FOXP3단백적표체정황,이급당피질격소치료적영향.방법 채용류식세포술검측47례SLE환자(기중29례위초발미경치료적활동기SLE)화22명정상인외주혈CD4+ CD25+ CD39+ T세포、CD4+CD25+ FOXP3+ T세포급CD4+ CD39+ FOXP3+ T세포백분솔이급FOXP3단백적표체,분석3조세포지간적상관성급당피질격소치료적영향.결과 SLE활동조、완해조、정상대조조외주혈CD4+ CD25+ CD39+ T세포백분솔분별위(1.3±0.5)%、(1.9±0.8)%、(2.3±1.0)%,해군세포재SLE활동조중적표체수평저우완해조화정상대조조(균P<0.05),이재후2조지간차이무통계학의의(P>0.05);SLE활동조중CD4+ CD25+、CD4+ CD25high급CD4+ CD39+ T세포표체적FOXP3단백백분솔분별위(45±12)%、(65±14)%、(70±14)%,FOXP3단백재CD4+ CD39+ T세포화CD4+ CD25highT세포중적표체수평명현고우재CD4+CD25+T세포중적표체수평(P<0.01),이재CD4+ CD39+T세포여CD4+CD25highT세포중적표체수평차이균무통계학의의(균P>0.05).결론 CD39가능시조절성T세포교호적표면표기,CD39+ Treg세포표체이상가능삼여SLE적발병궤제.
Objective To investigate the level of FOXP3 expressed in CD4+ CD39+ T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it . Methods Frequencies of CD4+ CD25+ CD39+, CD4+ CD25+ FOXP3+ and CD4+ CD39+ FOXP3+ T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results Percents of CD4+ CD25+ CD39+ T cells expressed in active SLE, inactive SLE and healthy controls were (1.3±0.5) %, (1.9±0.8) % and (2.3±1.0) % respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P<0.05 in each group, but it had no significant difference between the latter two groups (P>0.05). In active SLE, levels of FOXP3 protein expressed in CD4+ CD25+, CD4+ CD25high and CD4+ CD39+ T cells were (45±12)% ,(65±14)% and(70±14)% respectively. Levels of FOXP3 expressed in CD4+ CD25high and CD4+ CD39+ T cells were higher than that expressed in CD4+ CD25+ T cells (P<0.01), while it had no significant difference between CD4+ CD25highT cells and CD4+ CD39+ T cells (P>0.05). Conclusions These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39+ Treg cells may play an important role in the pathogenesis of SLE.