CAJ | 학술논문

用核酸原位杂交和用免疫组织化学法检测了6例豚鼠正常肺组织、10～18例癌旁正常肺组织和14例正常人外周血端粒酶逆转录酶(TERT) 亚基的mRNA和相应蛋白质表达.结果显示,豚鼠和人体正常肺组织支气管上皮细胞,肺泡巨噬细胞和少数肺泡上皮细胞有TERT mRNA和蛋白质表达.正常人外周血淋巴细胞中TERT原位杂交阳性表达细胞数为(17.26±8.57)%,免疫组织化学阳性细胞表达数为(28.10±16.78)%.提示:①正常肺组织支气管上皮细胞、肺泡巨噬细胞、少数肺泡上皮细胞和外周血淋巴细胞有TERT mRNA和蛋白质表达;②正常肺组织中上述细胞TERT mRNA和蛋白质的表达在生理状况下对于保证支气管上皮和肺泡上皮细胞再生,维持上皮组织的完整性可能起着重要的作用,同时有利于肺泡巨噬细胞发挥其肺内"卫士”功能;③淋巴细胞TERT表达对于维持机体的免疫功能可能有重要作用.
용핵산원위잡교화용면역조직화학법검측료6례돈서정상폐조직、10～18례암방정상폐조직화14례정상인외주혈단립매역전록매(TERT) 아기적mRNA화상응단백질표체.결과현시,돈서화인체정상폐조직지기관상피세포,폐포거서세포화소수폐포상피세포유TERT mRNA화단백질표체.정상인외주혈림파세포중TERT원위잡교양성표체세포수위(17.26±8.57)%,면역조직화학양성세포표체수위(28.10±16.78)%.제시:①정상폐조직지기관상피세포、폐포거서세포、소수폐포상피세포화외주혈림파세포유TERT mRNA화단백질표체;②정상폐조직중상술세포TERT mRNA화단백질적표체재생리상황하대우보증지기관상피화폐포상피세포재생,유지상피조직적완정성가능기착중요적작용,동시유리우폐포거서세포발휘기폐내"위사”공능;③림파세포TERT표체대우유지궤체적면역공능가능유중요작용.
In situ hybridization and immunohistostaining methods were employed to detect the expression of mRNA and protein of telomerase reverse transcriptase in normal lung tissue of 6 guinea pigs, adjacent normal tissues of 10-18 cases of carcinoma and circulating lymphocytesof 14 healthy subjects. The results showed that TERT mRNA and protein expression were detectable in epithelial cells of bronchi, pulmonary alveolar epithelial cells and alveolar macrophages of normal lung tissue in guinea pig and human body. In healthy subjects, the circulating lymphocytes positive for TERT expression were (17.26±8.57) % by in situ hybridization and (28.10±16.78) %, respectively. It was suggested that the expression of TERT mRNA and protein in some actively regenerating normal pulmonary cells might play a critical physiological role in keeping regeneration and integrality of airway epithelial tissue and pulmonary alveolar epithelium. It was also very important for alveolar macrophage to play as "safeguard” function in normal lung. The expression of TERT in circulating lymphocytes might play a critical role in maintaining normal human immunological function.