CAJ | 학술논문

采用L9 (315 )正交设计,研究了B5培养基营养组分对沙打旺组培根增殖的影响;并采用玻璃皿滤纸培养法,对其培养滤液提取物进行生物测定以验证沙打旺组培根的化感活性.结果显示:培养基的所有营养组分中,Fe2+对沙打旺组培根增殖的影响最大,蔗糖、H2PO+4、 Mg2+、 Mn2+、 Cu2+、 Zn2+、 BO3-3、 Co2+、 I-、C8H12ClNO3 + C12H18Cl2N4OS + C6H5O2N + C6H12O6的影响次之,氮、Ca2+、MoO2-4 和NAA的影响最小.根据不同养分条件下沙打旺组培根干重的极差分析,筛选出适宜沙打旺组培根快速增殖的优化培养基.培养滤液提取物的生物测定结果表明沙打旺组培根培养过程中可能产生化感物质;化感作用强度的差异预示营养胁迫可能影响其化感物质的产生.研究为沙打旺组培根再生与繁殖提供一定依据,并揭示养分条件可能是该植物表达化感作用的影响因素.
채용L9 (315 )정교설계,연구료B5배양기영양조분대사타왕조배근증식적영향;병채용파리명려지배양법,대기배양려액제취물진행생물측정이험증사타왕조배근적화감활성.결과현시:배양기적소유영양조분중,Fe2+대사타왕조배근증식적영향최대,자당、H2PO+4、 Mg2+、 Mn2+、 Cu2+、 Zn2+、 BO3-3、 Co2+、 I-、C8H12ClNO3 + C12H18Cl2N4OS + C6H5O2N + C6H12O6적영향차지,담、Ca2+、MoO2-4 화NAA적영향최소.근거불동양분조건하사타왕조배근간중적겁차분석,사선출괄의사타왕조배근쾌속증식적우화배양기.배양려액제취물적생물측정결과표명사타왕조배근배양과정중가능산생화감물질;화감작용강도적차이예시영양협박가능영향기화감물질적산생.연구위사타왕조배근재생여번식제공일정의거,병게시양분조건가능시해식물표체화감작용적영향인소.
The objective of this research was to determine effects of growth medium composition on the propagation of cultured milk vetch (Astragalus adsurgens Pall) roots. An orthogonal design was used to test multiple components in the growth medium. A second objective was to measure allelopathic activities of cultured milk vetch roots by conducting a bioassay using filtered extracts from the growth medium after culturing the roots. The results indicated that among all the nutritional components, Fe2+ had the greatest effect on the propagation of cultured milk vetch roots;the effects of sucrose,H2PO+4, Mg2+, Mn2+, Cu2+, Zn2+, BO3-3, Co2+, I-and C8H12ClNO3 + C12H18Cl2N4OS + C6H5O2N + C6H12O6 were intermediate;and the effects of nitrogen, Ca2+, MoO2-4 and NAA were lowest. A recommendation was made regarding the optimum nutrient content of growth medium for the propagation of cultured milk vetch roots. A bioassay using filtered extracts from the growth medium indicated that milk vetch roots might have produced allelopathic chemicals during their culture. Differences in the degree of allelopathic effects suggest that nutrient stress may influence the production of allelopathic chemicals. This study provides a basis for the improvement of milk root regeneration and propagation and suggests that nutrition may influence the production of allelopathic chemicals by this plant.