CAJ | 학술논문

目的探讨丹参酮ⅡA(TanⅡA)对谷氨酸(Glu)联合β淀粉样蛋白25-35 (Aβ_(25-35))诱导PC12细胞、Meynert核(nbM)神经元损伤的保护作用.方法培养PC12细胞,用MTT法观察TanⅡA三个不同浓度(2.5、5.0、10 μmol/L)对Glu联合Aβ_(25-35)损伤PC12细胞的保护作用;运用激光扫描共聚焦显微镜(LSCM)技术,检测急性分离nbM神经元在Glu联合Aβ诱导损伤时细胞内钙离子浓度([Ca~(2+)]_i)变化,及不同浓度TanⅡA对这种[Ca~(2+)]_i变化的影响.结果 MTT检测结果表明,TanⅡA 各组细胞活力、损伤程度与单纯Aβ+Glu损伤组相比有显著差异(P＜0.05),但无剂量依赖关系.Glu联合Aβ_(25-35)损伤组[Ca~(2+)]_i比对照组显著升高(P＜0.01);TanⅡA 各组[Ca~(2+)]_i均较单独Glu联合Aβ_(25-35)处理组有显著降低(P＜0.01).结论 TanⅡA通过提高细胞活力、维持细胞内钙离子浓度稳态以对抗Glu联合Aβ_(25-35)损伤作用.
목적 탐토단삼동ⅡA(TanⅡA)대곡안산(Glu)연합β정분양단백25-35 (Aβ_(25-35))유도PC12세포、Meynert핵(nbM)신경원손상적보호작용.방법 배양PC12세포,용MTT법관찰TanⅡA삼개불동농도(2.5、5.0、10 μmol/L)대Glu연합Aβ_(25-35)손상PC12세포적보호작용;운용격광소묘공취초현미경(LSCM)기술,검측급성분리nbM신경원재Glu연합Aβ유도손상시세포내개리자농도([Ca~(2+)]_i)변화,급불동농도TanⅡA대저충[Ca~(2+)]_i변화적영향.결과 MTT검측결과표명,TanⅡA 각조세포활력、손상정도여단순Aβ+Glu손상조상비유현저차이(P＜0.05),단무제량의뢰관계.Glu연합Aβ_(25-35)손상조[Ca~(2+)]_i비대조조현저승고(P＜0.01);TanⅡA 각조[Ca~(2+)]_i균교단독Glu연합Aβ_(25-35)처리조유현저강저(P＜0.01).결론 TanⅡA통과제고세포활력、유지세포내개리자농도은태이대항Glu연합Aβ_(25-35)손상작용.
Objective To explore the protection of TanshinoneⅡA (TanⅡA) on damage of PC12 cells and nucleus basalis of meynert (nbM) neurons induced by glutamate (Glu) and β-amyloid protein 25～35 (Aβ25 ～35). Methods MTT assay was used to observe the protection of different dosages of Tan ⅡA(2.5, 5.0, 10 μmol/L)on cellular damage induced by Glu and Aβ25-35. Laser scanning confocal microscopy (LSCM) technique was adopted to detect the change of the intracellular free calcium concentration ([Ca~(2+)]i). Results There were significant differences on the cell survival and the extent of damage between the each Tan ⅡA group and Aβ+Glu group (P＜0.05), but there wasn't dose-dependent manner. The level of [Ca~(2+)]i in Glu+Aβ25～35 group was significantly higher than that of control group (P＜0.01). The level of [Ca~(2+)]i in each Tan ⅡA goup was significantly lower than that of Glu+Aβ25-35 group(P＜0.01). Conclusions Tan ⅡA can be against the cellular damage induced by Glu and Aβ25-35 through increasing cell survival and sustaining the homeostasis of [Ca~(2+)]i.