中国矫形外科杂志
中國矯形外科雜誌
중국교형외과잡지
THE ORTHOPEDIC JOURNAL OF CHINA
2009年
21期
1643-1646
,共4页
侯克东%郭全义%张莉%袁玫%眭翔%汪爱媛%许文静%刘舒云%卢世璧
侯剋東%郭全義%張莉%袁玫%眭翔%汪愛媛%許文靜%劉舒雲%盧世璧
후극동%곽전의%장리%원매%휴상%왕애원%허문정%류서운%로세벽
干细胞%软骨细胞%细胞因子%生长因子%蛋白芯片法
榦細胞%軟骨細胞%細胞因子%生長因子%蛋白芯片法
간세포%연골세포%세포인자%생장인자%단백심편법
stem cells%chondrocyte%cytokines%growth factors%protein antibody array
[目的]观察从人脐带Wharton' s胶中分离的间质干细胞(WMSC)诱导分化成软骨细胞时表达细胞因子及生长因子的异同,从而为WMSC诱导的软骨细胞用于组织工程的构建以及其在临床的应用打下基础.[方法]从人正常足月分娩的脐带中分离间质干细胞,在体外以条件培基诱导分化成软骨细胞,免疫组化法鉴定其特性,应用细胞因子蛋白芯片方法检测WMSC及其诱导分化的软骨细胞所表达细胞因子及生长因子,并取成年人正常关节软骨为对照.[结果]78种细胞因子和生长因子在WMSC及其诱导分化的软骨细胞均有表达,两者表达谱相同,而表达量则多少有不同.诱导分化后表达量增多的因子为:MIP-3α,ENA-78,VEGF,PDGF-BB,SCF,FGF-7,GCP-2及SDF-1.减少的因子为MIP-1βHGF,NT-4,IL-10及MIP-δ.其共同表达的具免疫抑制作用的因子对降低异体移植时的免疫排斥可能有一定作用,其共同表达的金属蛋白酶抑制因子对其移植于体内时可具有保护作用.而共同表达的肿瘤生长抑制因子则可减少对干细胞移植后瘤变的担心.[结论]从人脐带wharton' s胶中分离的间质干细胞(WMSC)诱导分化成软骨细胞时表达细胞因子及生长因子谱相同,是一种理想的可替代自身软骨细胞的新型种子细胞,用于构建工程软骨组织.
[目的]觀察從人臍帶Wharton' s膠中分離的間質榦細胞(WMSC)誘導分化成軟骨細胞時錶達細胞因子及生長因子的異同,從而為WMSC誘導的軟骨細胞用于組織工程的構建以及其在臨床的應用打下基礎.[方法]從人正常足月分娩的臍帶中分離間質榦細胞,在體外以條件培基誘導分化成軟骨細胞,免疫組化法鑒定其特性,應用細胞因子蛋白芯片方法檢測WMSC及其誘導分化的軟骨細胞所錶達細胞因子及生長因子,併取成年人正常關節軟骨為對照.[結果]78種細胞因子和生長因子在WMSC及其誘導分化的軟骨細胞均有錶達,兩者錶達譜相同,而錶達量則多少有不同.誘導分化後錶達量增多的因子為:MIP-3α,ENA-78,VEGF,PDGF-BB,SCF,FGF-7,GCP-2及SDF-1.減少的因子為MIP-1βHGF,NT-4,IL-10及MIP-δ.其共同錶達的具免疫抑製作用的因子對降低異體移植時的免疫排斥可能有一定作用,其共同錶達的金屬蛋白酶抑製因子對其移植于體內時可具有保護作用.而共同錶達的腫瘤生長抑製因子則可減少對榦細胞移植後瘤變的擔心.[結論]從人臍帶wharton' s膠中分離的間質榦細胞(WMSC)誘導分化成軟骨細胞時錶達細胞因子及生長因子譜相同,是一種理想的可替代自身軟骨細胞的新型種子細胞,用于構建工程軟骨組織.
[목적]관찰종인제대Wharton' s효중분리적간질간세포(WMSC)유도분화성연골세포시표체세포인자급생장인자적이동,종이위WMSC유도적연골세포용우조직공정적구건이급기재림상적응용타하기출.[방법]종인정상족월분면적제대중분리간질간세포,재체외이조건배기유도분화성연골세포,면역조화법감정기특성,응용세포인자단백심편방법검측WMSC급기유도분화적연골세포소표체세포인자급생장인자,병취성년인정상관절연골위대조.[결과]78충세포인자화생장인자재WMSC급기유도분화적연골세포균유표체,량자표체보상동,이표체량칙다소유불동.유도분화후표체량증다적인자위:MIP-3α,ENA-78,VEGF,PDGF-BB,SCF,FGF-7,GCP-2급SDF-1.감소적인자위MIP-1βHGF,NT-4,IL-10급MIP-δ.기공동표체적구면역억제작용적인자대강저이체이식시적면역배척가능유일정작용,기공동표체적금속단백매억제인자대기이식우체내시가구유보호작용.이공동표체적종류생장억제인자칙가감소대간세포이식후류변적담심.[결론]종인제대wharton' s효중분리적간질간세포(WMSC)유도분화성연골세포시표체세포인자급생장인자보상동,시일충이상적가체대자신연골세포적신형충자세포,용우구건공정연골조직.
[Objective] To study cytokine and growth factor expression patterns of chondrocytes differentiated from mesen-chymal cells isolated from human umbilical cord Wharton's jelly and of normal adult articular chondrocytes.[Methods] Fresh umbilical cord were collected from healthy donor full - term births. Mesenchymal stem cells were isolated from Wharton's jelly of two human unbilical cords and cultured in conditional medium to differentiate to cartilage cells. Immunochemical staining method was used to identify the cartilage cell phenotype. Normal adult articular cartilage cells were isolated from one donor. Cytokine antibody - array technology (Clontech) was used to analyze cytokines and growth factors.[Results] The cytokine and growth factor profiles of chondrocytes differentiated from WMSC (Wharton jelly MSC) and normal cartilage cells were similar, but the expression level was somewhat different The maximum expression in chondrocyte were: MIP-3α, ENA -78, VEGF, PDGF -BB, SCF, FGF-7, GCP -2 and SDF-1. The decreased factors were : MIP-Iβ, HGF, NT-4, IL-10 and MIP-δ. A range of soluble immune tolerant factors IL - 10, TGF, VEGF and HGF were expressed in significant amount, thus creating a local immunosuppressive environment This result suggests that those factors may contribute to the ability of WMSC to avoid al-lorejection.WMSC and derived cartilage cell can produce oncostatin M and LIGHT, and high level of TIMP-1 and TIMP-2, which may help for prevent malignant change and enzyme digestion in vivo during transplantation. [ Conclusion ] These findings should encourage the use of mesenchymal stem cells from Wharton ' s jelly of human umbilical cord as a potential new seed cells in cartilage tissue engineering.