CAJ | 학술논문

目的应用线粒体单核苷酸多态性(SNPs)的复合PCR扩增体系应用于法医检验案件中的陈旧血痕、脱落细胞检材、腐败降解检材及骨组织等疑难检材,为解决法医学检验疑难检材的难题提供一种新的方法.方法用Identifiler~(TM)试剂盒进行目前使用的STR分型及已建立的18个线粒体SNPs(16个位于编码区和2个控制区)位点复合扩增毛细管电泳荧光检测体系,并行对照检验案件中的陈旧血痕、脱落细胞、腐败肌肉、软骨以及牙齿、骨等各种疑难检材.结果对于上述检材,STR与线粒体SNPs的检验成功率均达到80%以上,线粒体SNPs高于STR.对于高度腐败的肌肉及软骨、骨骼检材,线粒体SNPs的检验成功率明显高于STR.结论所建立的线粒体SNPs复合PCR扩增体系在疑难检材检验中具有良好的应用前景.
목적 응용선립체단핵감산다태성(SNPs)적복합PCR확증체계응용우법의검험안건중적진구혈흔、탈락세포검재、부패강해검재급골조직등의난검재,위해결법의학검험의난검재적난제제공일충신적방법.방법 용Identifiler~(TM)시제합진행목전사용적STR분형급이건립적18개선립체SNPs(16개위우편마구화2개공제구)위점복합확증모세관전영형광검측체계,병행대조검험안건중적진구혈흔、탈락세포、부패기육、연골이급아치、골등각충의난검재.결과 대우상술검재,STR여선립체SNPs적검험성공솔균체도80%이상,선립체SNPs고우STR.대우고도부패적기육급연골、골격검재,선립체SNPs적검험성공솔명현고우STR.결론 소건립적선립체SNPs복합PCR확증체계재의난검재검험중구유량호적응용전경.
Objective To provide a new means for forensic examination of difficult samples including old blood stain, hair (containing hair shafts), decomposed samples and old bone tissues using co-amplification of mitochondrial single nucleotide polymorphisms (SNPs). Methods With Identifiler~(TM) kit, STR genotyping and 18 mtDNA SNPs (16 SNPs in the coding region and 2 in the control region) detection were carried out using multiple amplification with labeled fluorescence and capillary electrophoresis. The two methods were compared for their performance in forensic caseworks such as old blood stain, cast-off cells, degraded muscles, cartilages, teeth and old bones. Results The detection rates of STR and mtDNA SNPs were both beyond 80%, but the latter had a greater success rate. The success rates of mtDNA SNPs were significantly greater than those of STR in the examination of such samples as degraded muscles, cartilages, and old bones. Conclusion Multiplex amplification of mitochondrial DNA SNPs shows a bright prospect in the examination of difficult forensic samples.