华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2010年
1期
9-12
,共4页
吲哚胺-2,3-双加氧酶%免疫耐受%非小细胞肺癌
吲哚胺-2,3-雙加氧酶%免疫耐受%非小細胞肺癌
신타알-2,3-쌍가양매%면역내수%비소세포폐암
Indoleamine-2,3-dioxygenase%immunotolerance%non-small cell lung cancer
目的 探讨吲哚胺-2,3-双加氧酶(indoleamine-2,3-dioxygenase,IDO)在非小细胞肺癌中的表达及意义.方法 收集38例非小细胞肺癌组织和10例癌旁正常肺组织.用半定量免疫组织化学方法 检测肺组织中IDO蛋白阳性率,实时荧光定量PCR检测肺组织中IDO mRNA表达水平.结果肺癌组IDO蛋白阳性率和IDO mRNA表达水平均明显高于对照组,差异均具有统计学意义(均P＜0.05);而肺癌组按不同病理分型中,腺癌组和鳞癌组比较差异无统计学意义(P＞0.05).肺癌组按不同分化程度分为高、中、低3组,经统计学分析,3组中IDO阳性表达率和IDO mRNA表达水平差异均有统计学意义(均P＜0.05).肺癌组按有和无淋巴结转移分组比较,IDO蛋白阳性率和IDO mRNA表达水平差异也均有统计学意义(均P＜0.05).结论 IDO在非小细胞肺癌中呈高表达,与分化程度及有无淋巴结转移有关,但与肺癌病理分型无关.
目的 探討吲哚胺-2,3-雙加氧酶(indoleamine-2,3-dioxygenase,IDO)在非小細胞肺癌中的錶達及意義.方法 收集38例非小細胞肺癌組織和10例癌徬正常肺組織.用半定量免疫組織化學方法 檢測肺組織中IDO蛋白暘性率,實時熒光定量PCR檢測肺組織中IDO mRNA錶達水平.結果肺癌組IDO蛋白暘性率和IDO mRNA錶達水平均明顯高于對照組,差異均具有統計學意義(均P＜0.05);而肺癌組按不同病理分型中,腺癌組和鱗癌組比較差異無統計學意義(P＞0.05).肺癌組按不同分化程度分為高、中、低3組,經統計學分析,3組中IDO暘性錶達率和IDO mRNA錶達水平差異均有統計學意義(均P＜0.05).肺癌組按有和無淋巴結轉移分組比較,IDO蛋白暘性率和IDO mRNA錶達水平差異也均有統計學意義(均P＜0.05).結論 IDO在非小細胞肺癌中呈高錶達,與分化程度及有無淋巴結轉移有關,但與肺癌病理分型無關.
목적 탐토신타알-2,3-쌍가양매(indoleamine-2,3-dioxygenase,IDO)재비소세포폐암중적표체급의의.방법 수집38례비소세포폐암조직화10례암방정상폐조직.용반정량면역조직화학방법 검측폐조직중IDO단백양성솔,실시형광정량PCR검측폐조직중IDO mRNA표체수평.결과폐암조IDO단백양성솔화IDO mRNA표체수평균명현고우대조조,차이균구유통계학의의(균P＜0.05);이폐암조안불동병리분형중,선암조화린암조비교차이무통계학의의(P＞0.05).폐암조안불동분화정도분위고、중、저3조,경통계학분석,3조중IDO양성표체솔화IDO mRNA표체수평차이균유통계학의의(균P＜0.05).폐암조안유화무림파결전이분조비교,IDO단백양성솔화IDO mRNA표체수평차이야균유통계학의의(균P＜0.05).결론 IDO재비소세포폐암중정고표체,여분화정도급유무림파결전이유관,단여폐암병리분형무관.
Objective To study the expression and significance of indoleamine-2,3-dioxygenase(IDO)in non-small-cell lung cancer(NSCLC).Methods Thirty-eight cases of lung cancer tissues and 10 cases of paracancerous normal lung tissue were collected.The semiquantitative immunohistochemistry was used to detect the positive rate of IDO and fluorescent quantitative PCR to detect the level of IDO mRNA in the lung tissues respectively.Results The positive rate of IDO and the level of IDO mRNA in NSCLC group were significantly higher than in paracancerous normal lung tissues(P＜0.05).There was no significant difference in the positive rate of IDO and the level of IDO mRNA between the adenocarcinoma group and the squamous cell carcinoma group(P＞0.05).Statistically,there was significant difference in positive rate of IDO and the level of IDO mRNA among low,middle and high differentiation groups(P＜0.05).There was significant difference in positive rate of IDO and the level of IDO mRNA between the lymph node metastasis and non-metastasis groups(P＜0.05).Conclusion The high expression of IDO in NSCLC is correlated with the degree of differentiation and the metastasis of lymph nodes,but not with the pathological types.
