CAJ | 학술논문

在7颗新鲜拔除的离体人恒牙釉质光滑面制备2 mm×3 mm实验窗,选用pH 4.5的化学酸蚀液在开窗区制备0,12,24,48,72,96,120 h不同时间的早期脱矿人工龋模型,采用自行研发的口腔光学CT系统进行人恒牙早期牙釉质人工龋的体外检出实验研究,设立同一牙齿样本的临床显微摄影和扫描电镜观测对照,以扫描电镜超微组织学检出结果为金标准,评价光学CT体外检出牙釉质早期脱矿的效能.口腔光学CT系统可同时无创获得牙齿表面和截面的脱矿信息,准确检测到脱矿12 h以内微米量级的早期牙釉质表面组织学水平的改变,早于裸眼观察和临床显微摄影.光学CT对于不同酸蚀期实验窗早期脱矿的检出与扫描电镜表征完全一致.口腔光学CT可以牙齿表面快速扫描的方式,非接触、准确、量化地检出牙釉质早期人工脱矿,检出敏感度高,重复性好,可无创获得脱矿部位微米量级量化分析数据.
재7과신선발제적리체인항아유질광활면제비2 mm×3 mm실험창,선용pH 4.5적화학산식액재개창구제비0,12,24,48,72,96,120 h불동시간적조기탈광인공우모형,채용자행연발적구강광학CT계통진행인항아조기아유질인공우적체외검출실험연구,설립동일아치양본적림상현미섭영화소묘전경관측대조,이소묘전경초미조직학검출결과위금표준,평개광학CT체외검출아유질조기탈광적효능.구강광학CT계통가동시무창획득아치표면화절면적탈광신식,준학검측도탈광12 h이내미미량급적조기아유질표면조직학수평적개변,조우라안관찰화림상현미섭영.광학CT대우불동산식기실험창조기탈광적검출여소묘전경표정완전일치.구강광학CT가이아치표면쾌속소묘적방식,비접촉、준학、양화지검출아유질조기인공탈광,검출민감도고,중복성호,가무창획득탈광부위미미량급양화분석수거.
2 mmx3 mm experimental windows were prepared in adamant slippery surfaces of 7 fresh uprooted permanent teeth. The teeth surfaces in the windows area were demineralized to create artificial caries mould of early stage by aciding the experimental teeth surfaces of 0, 12, 24, 48, 72, 96, 120 hours with demineralized liquid (pH 4.5) in vitro. The demineralized changes on the experimental teeth surfaces were detected by dental Optical Coherence Tomography (OCT) system, which were newly developed by our research group recently, and the detecting results were compared with clinical digital photomicrography and scanning electron microscopy on the same tooth sample, to checkout the efficacy and feasibility of dental OCT for early quantification detecting of artificial enamel demineralization in vitro. The dental OCT system can safelydetect early enamel demineralization of micron level and noninvasively obtain fine resolution quantification information both in surfaces view and sectional view; OCT could accurately detect surface demineralization changes on the experimental windows of artificial dental caries as early as after 12 hours aciding treatment, earlier than the visual inspection and clinical digital photomicrography. OCT could obtain both superficial view and sectional view of quantificational demineralization in early enamel caries homeochronously, and had high correlation to the results of ultramicromorphological changes detected by scanning electron microscopy. Dental OCT system developed by our group could accurately detect early artificial dental caries atraumaticly with high sensitivity and safety. Moreover, it can obtain quantification data in micron level without damaging the experimental teeth samples.