医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2010年
2期
109-114
,共6页
黄清玲%郑大利%林建银%林旭
黃清玲%鄭大利%林建銀%林旭
황청령%정대리%림건은%림욱
乙型肝炎病毒%基质金属蛋白酶%组织金属蛋白酶抑制剂
乙型肝炎病毒%基質金屬蛋白酶%組織金屬蛋白酶抑製劑
을형간염병독%기질금속단백매%조직금속단백매억제제
hepatitis B virus%matrix metalloproteinases%tissue inhibitors of metalloproteinases
目的 研究HBV全长对HepG2细胞侵袭相关基因表达及活性的影响,探讨HBV在整体水平对HepG2细胞侵袭的影响.方法 采用定量PCR分析HBV对HepG2细胞MMP2、9和TIMP1-4基因转录的影响;通过明胶酶谱及反相明胶酶谱检测MMP2、MMP9及TIMPs的活性;应用体外侵袭小室法检测细胞的侵袭能力.结果 HBV的复制可以促进HepG2细胞MMP2、MMP9、TIMP1和TIMP3基因的转录,抑制TIMP4基因转录,增强HepG2细胞MMP2 、MMP9的活性并增强细胞中TIMP1、TIMP3功能,HBV稳定复制的细胞具有更强的体外侵袭能力.结论 HBV可影响HepG2细胞MMPs和TIMPs的基因转录、表达及功能,促进HepG2细胞的体外侵袭,这可能与HBV相关的HCC侵袭转移密切相关.
目的 研究HBV全長對HepG2細胞侵襲相關基因錶達及活性的影響,探討HBV在整體水平對HepG2細胞侵襲的影響.方法 採用定量PCR分析HBV對HepG2細胞MMP2、9和TIMP1-4基因轉錄的影響;通過明膠酶譜及反相明膠酶譜檢測MMP2、MMP9及TIMPs的活性;應用體外侵襲小室法檢測細胞的侵襲能力.結果 HBV的複製可以促進HepG2細胞MMP2、MMP9、TIMP1和TIMP3基因的轉錄,抑製TIMP4基因轉錄,增彊HepG2細胞MMP2 、MMP9的活性併增彊細胞中TIMP1、TIMP3功能,HBV穩定複製的細胞具有更彊的體外侵襲能力.結論 HBV可影響HepG2細胞MMPs和TIMPs的基因轉錄、錶達及功能,促進HepG2細胞的體外侵襲,這可能與HBV相關的HCC侵襲轉移密切相關.
목적 연구HBV전장대HepG2세포침습상관기인표체급활성적영향,탐토HBV재정체수평대HepG2세포침습적영향.방법 채용정량PCR분석HBV대HepG2세포MMP2、9화TIMP1-4기인전록적영향;통과명효매보급반상명효매보검측MMP2、MMP9급TIMPs적활성;응용체외침습소실법검측세포적침습능력.결과 HBV적복제가이촉진HepG2세포MMP2、MMP9、TIMP1화TIMP3기인적전록,억제TIMP4기인전록,증강HepG2세포MMP2 、MMP9적활성병증강세포중TIMP1、TIMP3공능,HBV은정복제적세포구유경강적체외침습능력.결론 HBV가영향HepG2세포MMPs화TIMPs적기인전록、표체급공능,촉진HepG2세포적체외침습,저가능여HBV상관적HCC침습전이밀절상관.
Objective To explore the effect of HBV on expression and activity of invasion-relative genes and invasion ability of HepG2 cells.Methods Human hepatoblastoma HepG2 cell line harboring 1.2 unit-length of HBV genome or containing only empty vector as control was used. The expression and activity of MMP2,MMP9,TIMP1,2,3,and 4 were detected by real-time PCR,gelatin zymography or reverse zymography. In vitro invasion ability was measured by Matrigel transwell invasion assay.Results The replication of full-length of HBV genome up-regulated expression of MMP2,MMP9,TIMP1,TIMP4,down-regulated expression of TIMP4,increased activity of MMP2,MMP9,TIMP1,TIMP3,and then promoted invasion ability of HepG2 cells,as compared with the empty vector control.Conclusion HBV genome influences invasion ability of HepG2 cells by deregulation of MMPs and TIMPs replication,which may provide new insights into the role of HBV in metastasis of hepatocellular carcinoma.
