CAJ | 학술논문

分别采用肠球菌选择性培养基(EC)从健康婴儿的粪便、健康仔猪的肠道及粪便中分离筛选了4株屎肠球菌,分别命名为EfB、Ef1-2、Ef3-4、Ef4-1,利用Biolog自动微生物分析系统鉴定结果表明,它们都是屎肠球菌,而且经16s rRNA基因序列测序比较分析,表明这4株菌与数据库中已发表的屎肠球菌16s rRNA序列(AccessionNo.AY675247)同源性均为99%,两者鉴定结果一致.试验表明,所筛得菌株生长速度较快,且对致病型大肠杆菌K88,K99,金黄色葡萄球菌,巴氏杆菌及沙门氏菌都有较强的抑制作用,其中对大肠杆菌K99的抑菌效果最好.屎肠球菌新菌种的鉴定与筛选为开发新型微生态类饲料添加剂提供了帮助.
분별채용장구균선택성배양기(EC)종건강영인적분편、건강자저적장도급분편중분리사선료4주시장구균,분별명명위EfB、Ef1-2、Ef3-4、Ef4-1,이용Biolog자동미생물분석계통감정결과표명,타문도시시장구균,이차경16s rRNA기인서렬측서비교분석,표명저4주균여수거고중이발표적시장구균16s rRNA서렬(AccessionNo.AY675247)동원성균위99%,량자감정결과일치.시험표명,소사득균주생장속도교쾌,차대치병형대장간균K88,K99,금황색포도구균,파씨간균급사문씨균도유교강적억제작용,기중대대장간균K99적억균효과최호.시장구균신균충적감정여사선위개발신형미생태류사료첨가제제공료방조.
Four new strains of Enterococcus faecium were screened from healthy infant feces,piglet intestine and feces in EC medium,which were named as EfB,Ef1-2,Ef3-4 and Ef4-1.They were all identified as Enterococcus faecium by Biolog automatic microbe analysis system.16S rRNA identification comparing with GenBank indicated thatthere was 99%homology with Enterococcus faecium strain SF(Accession No.AY675247).Results:All the four new strains have faster growth rate and can significantly inhibit growth of Eseherichia coli K88,Escherichia coli K99,Staphylococcus Aureus,Pasteurellosis Bacillus and Salmonella,especially Escherichia coli K99.The identification results with two ways are consistent.The screening,identification and development of new Enterococcus faecium would be ben-eficial for the development of micro-ecological agents for feed use.