CAJ | 학술논문

利用热回流法和超声波法提取细果角茴香中的总黄酮，结合NaNO2-Al（NO3）3-NaOH体系分光光度法测定细果角茴香中的总黄酮含量，并对细果角茴香中总黄酮的提取方法进行优化。结果表明：利用超声波法提取细果角茴香中的总黄酮效果较好；且在甲醇浓度为70％、料液比为1：20、超声波提取2次、提取时间分别为2．0h和1．5h的条件下提取效果最好，测得总黄酮的含量为15．44mg／g。NaNO2-Al（NO3）3-NaOH体系分光光度法操作简捷，测定结果准确可靠。
이용열회류법화초성파법제취세과각회향중적총황동，결합NaNO2-Al（NO3）3-NaOH체계분광광도법측정세과각회향중적총황동함량，병대세과각회향중총황동적제취방법진행우화。결과표명：이용초성파법제취세과각회향중적총황동효과교호；차재갑순농도위70％、료액비위1：20、초성파제취2차、제취시간분별위2．0h화1．5h적조건하제취효과최호，측득총황동적함량위15．44mg／g。NaNO2-Al（NO3）3-NaOH체계분광광도법조작간첩，측정결과준학가고。
Total flavonoids in H. leptocarpum was extracted by hot reflux method and ultrasonic extraction method, and its content was determined by NaNO2-Al （NO3 ）3-NaOH spectrometry. The results showed that ultrasonic method was more suitable than hot reflux method, and the optimum extraction condition was 70% methanol solution, solid-liquid ratio 1：20 （W/V）, ultrasonic treatment twice with the time of 2. 0 h and 1.5 h. Total flavonoids content in H. leptocarpum was about 15.44 mg/g. NaNO2-Al （NO3 ） 3-NaOH spectrometry method was easy to operate and the results were accurate.