中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
3期
288-290
,共3页
徐青果%王成夭%彭勉%刘薇
徐青果%王成夭%彭勉%劉薇
서청과%왕성요%팽면%류미
认知功能障碍%连接蛋白类%海马%老年人
認知功能障礙%連接蛋白類%海馬%老年人
인지공능장애%련접단백류%해마%노년인
Cognitive dysfunction%Connexins%Hippocampus%Aged
目的 评价术后认知功能障碍老龄大鼠海马神经元缝隙连接蛋白36(Cx36)表达的变化.方法 健康雄性SD大鼠90只,20月龄,体重500～600 g,按随机数字表法,将其随机分为3组(n=30):正常对照组(C组)、假手术组(S组)和术后认知功能障碍组(POCD组).采用脾脏切除术制备老龄大鼠术后认知功能障碍模型,分别于脾脏切除术后1、3、7 d(T1-3)时每组各取10只大鼠,经旷场分析和Y型迷宫实验测试大鼠认知功能,随后处死大鼠取海马,采用免疫组化法检测海马神经元Cx36的表达.结果 与C组比较,S组各项认知功能指标和海马神经元Cx36表达水平差异无统计学意义(P＞0.05),POCD组中央格停留时间延长,跨格次数、站立次数和正确反应次数减少,全天总反应时间增加,海马神经元Cx36表达水平降低(P＜0.05)；与S组比较,POCD组大鼠T1时中央格停留时间延长,跨格次数、站立次数和正确反应次数减少,全天总反应时间增加,海马神经元Cx36表达水平降低(P＜0.05),T2,3时各项认知功能指标及海马神经元Cx36表达水平差异无统计学意义(P＞0.05).结论 术后认知功能障碍可能与老龄大鼠海马神经元Cx36表达下调有关.
目的 評價術後認知功能障礙老齡大鼠海馬神經元縫隙連接蛋白36(Cx36)錶達的變化.方法 健康雄性SD大鼠90隻,20月齡,體重500～600 g,按隨機數字錶法,將其隨機分為3組(n=30):正常對照組(C組)、假手術組(S組)和術後認知功能障礙組(POCD組).採用脾髒切除術製備老齡大鼠術後認知功能障礙模型,分彆于脾髒切除術後1、3、7 d(T1-3)時每組各取10隻大鼠,經曠場分析和Y型迷宮實驗測試大鼠認知功能,隨後處死大鼠取海馬,採用免疫組化法檢測海馬神經元Cx36的錶達.結果 與C組比較,S組各項認知功能指標和海馬神經元Cx36錶達水平差異無統計學意義(P＞0.05),POCD組中央格停留時間延長,跨格次數、站立次數和正確反應次數減少,全天總反應時間增加,海馬神經元Cx36錶達水平降低(P＜0.05)；與S組比較,POCD組大鼠T1時中央格停留時間延長,跨格次數、站立次數和正確反應次數減少,全天總反應時間增加,海馬神經元Cx36錶達水平降低(P＜0.05),T2,3時各項認知功能指標及海馬神經元Cx36錶達水平差異無統計學意義(P＞0.05).結論 術後認知功能障礙可能與老齡大鼠海馬神經元Cx36錶達下調有關.
목적 평개술후인지공능장애노령대서해마신경원봉극련접단백36(Cx36)표체적변화.방법 건강웅성SD대서90지,20월령,체중500～600 g,안수궤수자표법,장기수궤분위3조(n=30):정상대조조(C조)、가수술조(S조)화술후인지공능장애조(POCD조).채용비장절제술제비노령대서술후인지공능장애모형,분별우비장절제술후1、3、7 d(T1-3)시매조각취10지대서,경광장분석화Y형미궁실험측시대서인지공능,수후처사대서취해마,채용면역조화법검측해마신경원Cx36적표체.결과 여C조비교,S조각항인지공능지표화해마신경원Cx36표체수평차이무통계학의의(P＞0.05),POCD조중앙격정류시간연장,과격차수、참립차수화정학반응차수감소,전천총반응시간증가,해마신경원Cx36표체수평강저(P＜0.05)；여S조비교,POCD조대서T1시중앙격정류시간연장,과격차수、참립차수화정학반응차수감소,전천총반응시간증가,해마신경원Cx36표체수평강저(P＜0.05),T2,3시각항인지공능지표급해마신경원Cx36표체수평차이무통계학의의(P＞0.05).결론 술후인지공능장애가능여노령대서해마신경원Cx36표체하조유관.
Objective To investigate the changes in expression of hippocampal neuronal gap function protein connexin 36 (Cx36) induced by postoperative cognitive dysfunction (POCD) in aged rats.Methods Ninety male SD rats aged 20 months weighing 500-600 g were randomly divided into 3 groups ( n =30 each):group normal control (group C); group sham operation (group S) and group POCD.POCD was induced by splenectomy.Cognitive function was assessed by using open field test and maze test on the 1st,3rd and 7th day after operation (T1,T2,T3 ).The ultrastructure of gap junction in the CA1 region of hippocampas was examined with thin-section transmission electron microscope.The expression of Cx36 was detected by immuno-histochemical method.Results POCD significantly decreased the number of grid cross,the rearing and correct responses and increased the time the animab spent in the central square and total reaction time at T1 in group POCD as compared with group C.Cx36 expression was significantly decreased at T1 in group POCD as compared with group C.The ultrastructure of gap junctions underwent significant change at T1 in group POCD.Conclusion Hippocampal neuronal Cx36 may be involved in the cognitive dysfunction after splenectomy in aged rats.
