CAJ | 학술논문

目的探讨山东地区结核分枝杆菌临床分离菌株的基因型特征,评估不同基因型分布对于耐药结核菌近期传播的影响.方法在山东地区选取13个结核病防治机构作为监测哨点收集临床分离菌株和相关信息,应用分枝杆菌散在重复单位(MIRU)技术分析结核分枝杆菌DNA多态性.结果 1年的研究期内共获得558株结核分枝杆菌,对12个MIRU位点进行检测共产生143个基因型,其中成簇基因型66个,成簇率86.2%.74.6%的菌株属于北京家族,177个(31.7%)菌株属于山东基因型.耐多药菌株的近期感染估计值明显低于敏感菌株.结论山东地区结核分枝杆菌具有明显的基因多态性,山东基因型菌株在人群中具有较强的传播能力.
목적 탐토산동지구결핵분지간균림상분리균주적기인형특정,평고불동기인형분포대우내약결핵균근기전파적영향.방법 재산동지구선취13개결핵병방치궤구작위감측초점수집림상분리균주화상관신식,응용분지간균산재중복단위(MIRU)기술분석결핵분지간균DNA다태성.결과 1년적연구기내공획득558주결핵분지간균,대12개MIRU위점진행검측공산생143개기인형,기중성족기인형66개,성족솔86.2%.74.6%적균주속우북경가족,177개(31.7%)균주속우산동기인형.내다약균주적근기감염고계치명현저우민감균주.결론 산동지구결핵분지간균구유명현적기인다태성,산동기인형균주재인군중구유교강적전파능력.
Objective To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong. Methods Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units (MIRU) methods. Results 12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns,and 481 (86.2%) strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level,comparing to the susceptible strains. Conclusion Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.