CAJ | 학술논문

目的探讨抑制内源性微小RNA-375(miR-375)是否影响NIT-1胰岛β细胞的脂性凋亡.方法将NIT-1细胞分为4组:空白组(无脂质体)、空转组(脂质体)、阴性对照miRNA组(脂质体+阴性对照miRNA)、2'-O-me-375组(脂质体+2'-O-me-375,抑制内源性miR-375的作用).转染72 h后,各组细胞分别以500 μmol/L棕榈酸孵育48 h.以Hochest 33342染色和脱氧核糖核酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡,以Western印迹法检测miR-375靶基因肌营养素(myotrophin,VI)的表达.结果与其他3组比较,2'-O-me-375 RNA组NIT-1脂性凋亡率最低(P<0.01),同时V1表达水平最高(P<0.01).结论抑制内源性miR-375可降低胰岛β细胞的脂性凋亡.
목적 탐토억제내원성미소RNA-375(miR-375)시부영향NIT-1이도β세포적지성조망.방법 장NIT-1세포분위4조:공백조(무지질체)、공전조(지질체)、음성대조miRNA조(지질체+음성대조miRNA)、2'-O-me-375조(지질체+2'-O-me-375,억제내원성miR-375적작용).전염72 h후,각조세포분별이500 μmol/L종려산부육48 h.이Hochest 33342염색화탈양핵당핵산말단전이매개도적결구말단표기법(TUNEL)검측세포조망,이Western인적법검측miR-375파기인기영양소(myotrophin,VI)적표체.결과 여기타3조비교,2'-O-me-375 RNA조NIT-1지성조망솔최저(P<0.01),동시V1표체수평최고(P<0.01).결론 억제내원성miR-375가강저이도β세포적지성조망.
Objective To investigate the effect of miR-375 inhibited by 2'-O-me-375 on lipoapoptosis of NIT-1 pancreatic β cells. Methods NIT-1 cells were divided and treated according to the optimal condition: mock (without lipofectamine) ,lipofectamine( transfected only with lipofectamine) ,NC-miRNA (transfected with negative control miRNA) ,and 2'-O-me-375( transfected with 2'-O-me-375) groups. 72 hours later, all cells in each group were cultured with 500 μmol/L palmitate for 48 h. The percentage of apoptotic cells was detected by Hochest33342 staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL). The protein expression of myotrophin ( V1 ) , a target gene of miR-375, was detected by Western blotting. Results Compared to the other three groups,the cell apoptosis rate of 2'-O-me-375 group was the lowest (P<0.01) .along with the highest VI expression level(P<0. 01). Conclusion Inhibition of miR-375 decreases pancreatic (3-cell lipoapoptosis.