CAJ | 학술논문

目的观察乌司他丁(天普洛安)对大鼠放射性肺损伤过程中TNF-α及IL-6的影响,试图寻找一种预防或治疗放射性肺损伤的有效途径.方法 72只雌性SD大鼠随机分为3组:健康对照组、单纯照射组和乌司他丁治疗组,每组24只.照射组及治疗组动物麻醉后,行直线加速器全胸部照射一次,剂量为25 Gy.照射组照射后通过尾静脉注射乌司他丁(100 000 U·kg-1·d-1),共计7 d.对照组和照射组注射同等体积的生理盐水.于照射后2 h、4、8和24周处死动物,取部分肺组织行HE染色及Masson染色,观察组织学变化,另提取组织蛋白用免疫印迹杂交方法检测肺组织中TNF-α水平,使用酶联免疫吸附分析法检测血清中IL-6水平.所有数据采用SPSS统计软件进行方差分析.结果照射组大鼠肺组织中TNF-α及血清中IL-6水平与对照组相比,明显升高,并在第4周时达到顶峰(q=5.63、6.21,P＜0.01);治疗组TNF-α和IL-6水平与对照组相比,第4周时也增加明显,但是与照射组相比明显下降(q=4.97、7.42,P＜0.01).结论大鼠放射性肺损伤时TNF-α及IL-6水平明显升高,在放射性肺损伤发展中起到重要作用,乌司他丁能显著降低其水平,并抑制炎性反应,从而能有效地防治放射性肺损伤.
목적 관찰오사타정(천보락안)대대서방사성폐손상과정중TNF-α급IL-6적영향,시도심조일충예방혹치료방사성폐손상적유효도경.방법 72지자성SD대서수궤분위3조:건강대조조、단순조사조화오사타정치료조,매조24지.조사조급치료조동물마취후,행직선가속기전흉부조사일차,제량위25 Gy.조사조조사후통과미정맥주사오사타정(100 000 U·kg-1·d-1),공계7 d.대조조화조사조주사동등체적적생리염수.우조사후2 h、4、8화24주처사동물,취부분폐조직행HE염색급Masson염색,관찰조직학변화,령제취조직단백용면역인적잡교방법검측폐조직중TNF-α수평,사용매련면역흡부분석법검측혈청중IL-6수평.소유수거채용SPSS통계연건진행방차분석.결과 조사조대서폐조직중TNF-α급혈청중IL-6수평여대조조상비,명현승고,병재제4주시체도정봉(q=5.63、6.21,P＜0.01);치료조TNF-α화IL-6수평여대조조상비,제4주시야증가명현,단시여조사조상비명현하강(q=4.97、7.42,P＜0.01).결론 대서방사성폐손상시TNF-α급IL-6수평명현승고,재방사성폐손상발전중기도중요작용,오사타정능현저강저기수평,병억제염성반응,종이능유효지방치방사성폐손상.
Objective To observe the effect of ulinastatin on tumor necrosis factor(TNF-α)and interleukin-6(IL-6)in radiation-induced lung injury.Methods Severity-two female SD rats were randomly divided into 3 groups as control group,irradiation group and treatment group(administered with Ulinastatin).Rats in irradiation group and treatment group were irradiated with linear accelerator at a single dose of 25 Gy.After irradiation rats in treatment group were injected daily with ulinastatin at a dose of 100000 U-kg-1·d-1 for 7 days through caudal vein while rats in control group and irradiation group were injected with the same volume of saline.Rats were killed at 2 h,4,8 and 24 weeks.Samples of lung tissues were observed by using HE staining.Expression of TNF-α in lung was determined by Western blot and expression of IL-6 in serum was determined by ELISA.Data were analyzed by SPSS software.Results Expressions of TNF-α in lung and IL-6 in serum increased significantly after irradiated in irradiation group compared with control group,and it reached the peak at 4 weeks(q=5.63、6.21,P＜0.01).Though expressions of TNF-α and IL-6 in ffeatment group also increased compared with control group,the difference between irradiation group and treatment group was statistic significantly(q=4.97、7.42,P＜0.01).Conclusions TNF-α and IL-6 play an important role in radiation-induced lung injury.Ulinastatin could suppress the inflammatory response and radiation-induced lung injury effectively by decreasing the levels of TNF-α and IL-6.