CAJ | 학술논문

目的探讨肺癌细胞MICA/B分子表达与纵隔淋巴结转移的相关性.方法采集30例肺癌患者手术切除组织标本,新鲜肺癌组织作为试验组,癌旁组织作为对照组,采用流式细胞术测定肺癌细胞表面MICA/B分子的表达,全部患者术中系统清扫淋巴结作病理检查,根据有无淋巴结转移分成N0、N1、N23组,比较3组MICA/B分子的表达情况.结果肺癌组织细胞表面MICA/B表达阳性率(0.3788±0.2398)%,与癌旁组织的(0.1908±0.1760)%比较,差异有统计学意义(P＜0.01).N0组与N1组、N1组与N2组MICA/B表达差异无统计学意义(P＞0.05),N0组与N2组MICA/B表达差异有统计学意义(P＜0.05).结论 MICA/B高表达于肺癌组织细胞表面.肺癌纵隔淋巴结转移时,MICA/B表达明显增高,预后较差.MICA/B表达可作为发生纵隔淋巴结转移的一个指标.
목적 탐토폐암세포MICA/B분자표체여종격림파결전이적상관성.방법 채집30례폐암환자수술절제조직표본,신선폐암조직작위시험조,암방조직작위대조조,채용류식세포술측정폐암세포표면MICA/B분자적표체,전부환자술중계통청소림파결작병리검사,근거유무림파결전이분성N0、N1、N23조,비교3조MICA/B분자적표체정황.결과 폐암조직세포표면MICA/B표체양성솔(0.3788±0.2398)%,여암방조직적(0.1908±0.1760)%비교,차이유통계학의의(P＜0.01).N0조여N1조、N1조여N2조MICA/B표체차이무통계학의의(P＞0.05),N0조여N2조MICA/B표체차이유통계학의의(P＜0.05).결론 MICA/B고표체우폐암조직세포표면.폐암종격림파결전이시,MICA/B표체명현증고,예후교차.MICA/B표체가작위발생종격림파결전이적일개지표.
Objective To investigate the relation between the expression of MICA/B in lung cancer cells and the mediastinum lymph node metastasis. Methods The samples of the lung cancer tissue as test group and the healthy tissue beside lung cancer as control group from 30 cases of patients with lung cancer were collected, and the expression of MICA/B on lung cancer cells surface were detected by flow cytometry.All patients were divided into three groups(N0, N1, N2) according to the state of lymph node metastasis, and the expression of MICA/B was analyzed among the three groups. Results The expression level of MICA/B in test group was significantly higher than that in control group[(0.3788±0.2398) %, (0.1908±0.1760) %] (P ＜0.01),however the MICA/B expression level between N0 and N1 or between N1 and N2 was not statistically different (P＞0.05), while that between N0 and N2 had statistical difference (P＜0.05). Conclusion The expression level of MICA/B on surface of lung cancer cells is high, and the MICA/B as ligand of NKG2D may play an important role in the tumor immune response. The expression of MICA/B in mediastinum metastatic lymph node from lung cancer is remarkably increased and the prognosis of patients with lymph node metastasis is poor. MICA/B could be considered as a marker of mediastinum lymph node metastasis.