CAJ | 학술논문

目的探讨CliniMACS系统进行CD+34造血干细胞阳性富集的有效性、安全性及应用效果.方法对39例实体瘤和自身免疫性疾病患者动员后采集的外周血干细胞进行了CliniMACS系统阳性富集的CD+34自体造血干细胞移植.结果阳性富集后CD+34细胞纯度平均可达89.8%,CD+34细胞平均回收率达78.9%,粒细胞-巨噬细胞集落形成单位(CFU-GM)数量为(15.0±7.0)×105/kg,阳性富集前、后细胞胎盘蓝拒染率比较差异无统计学意义,保持了细胞良好的生物学活性,移植后于2～3周内造血重建.结论应用CliniMACS系统进行CD+34造血干细胞阳性富集,可安全、有效地应用于造血干细胞移植.
목적 탐토CliniMACS계통진행CD+34조혈간세포양성부집적유효성、안전성급응용효과.방법 대39례실체류화자신면역성질병환자동원후채집적외주혈간세포진행료CliniMACS계통양성부집적CD+34자체조혈간세포이식.결과 양성부집후CD+34세포순도평균가체89.8%,CD+34세포평균회수솔체78.9%,립세포-거서세포집락형성단위(CFU-GM)수량위(15.0±7.0)×105/kg,양성부집전、후세포태반람거염솔비교차이무통계학의의,보지료세포량호적생물학활성,이식후우2～3주내조혈중건.결론 응용CliniMACS계통진행CD+34조혈간세포양성부집,가안전、유효지응용우조혈간세포이식.
Objective To investigate the validity and security of CD+34 hematopoietic stem cells enrichment by using CliniMACS device. Methods Thirty-nine patients with solid tumor and autoimmune disease received CD+34 HSCT, the CD+34 cells were enriched by CliniMACS device. Results After enrichment, the median purity of CD+34 cells was 89.8%, the median recovery rate of CD+34 cells was 78.9%, the granulocyte-macrophage cluster-colony forming unit(CFU-GM) counts were(15.0±7.0)×105/kg. The refuse rate of typan blue was similar, compairing before and after enrichment, and the hematopoietic function recovers 2-3 weeks after transplantation. Conclusions The feasibility and safety of the CD+34 cells enrichment by using CliniMACS device in patient before HSCT were confirmed.