中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2009年
9期
537-539
,共3页
付妤%童晶晶%潘琦%王文峰%邹开芳%钱伟%侯晓华
付妤%童晶晶%潘琦%王文峰%鄒開芳%錢偉%侯曉華
부여%동정정%반기%왕문봉%추개방%전위%후효화
结肠炎%溃疡性%T淋巴细胞%辅助诱导%白细胞介素-17
結腸炎%潰瘍性%T淋巴細胞%輔助誘導%白細胞介素-17
결장염%궤양성%T림파세포%보조유도%백세포개소-17
Colitis,ulcerative%T lymphocytes,Helper inducer%Interleukin-17
目的 探讨Th细胞各亚群比例在溃疡性结肠炎(UC)发病中的变化及意义.方法 按照2007年济南标准收集UC患者20例,对照组16例.利用细胞内细胞因子染色和四色荧光抗体流式细胞术对肠黏膜及外周血淋巴细胞作表型分析,比较各组肠黏膜固有层单个核细胞(cLPMC)和外周血单个核细胞(PBMC)中Th1、Th2、Th17细胞比例的改变.结果 UC组cLPMC中Th17细胞比例较对照组升高,分别为3.75%(6.93%)和1.25%(3.70%),在PBMC中为1.40%(2.15%)和0.70%(0.33%),两组间差异均有统计学意义(P值均<0.05).UC患者CLPMC中Th17细胞比例与疾病评分呈正相关(r=0.34,P<0.05).UC组和对照组Th1、Th2细胞在cLPMC和PBMC中的比例均差异无统汁学意义(P>0.05),且不同疾病活动度患者间差异亦无统计学意义(P值均>0.05).结论 在Th细胞各亚群中,Th17细胞是介导UC肠道局部和外周免疫应答的优势细胞,可能成为UC治疗的有效靶点.
目的 探討Th細胞各亞群比例在潰瘍性結腸炎(UC)髮病中的變化及意義.方法 按照2007年濟南標準收集UC患者20例,對照組16例.利用細胞內細胞因子染色和四色熒光抗體流式細胞術對腸黏膜及外週血淋巴細胞作錶型分析,比較各組腸黏膜固有層單箇覈細胞(cLPMC)和外週血單箇覈細胞(PBMC)中Th1、Th2、Th17細胞比例的改變.結果 UC組cLPMC中Th17細胞比例較對照組升高,分彆為3.75%(6.93%)和1.25%(3.70%),在PBMC中為1.40%(2.15%)和0.70%(0.33%),兩組間差異均有統計學意義(P值均<0.05).UC患者CLPMC中Th17細胞比例與疾病評分呈正相關(r=0.34,P<0.05).UC組和對照組Th1、Th2細胞在cLPMC和PBMC中的比例均差異無統汁學意義(P>0.05),且不同疾病活動度患者間差異亦無統計學意義(P值均>0.05).結論 在Th細胞各亞群中,Th17細胞是介導UC腸道跼部和外週免疫應答的優勢細胞,可能成為UC治療的有效靶點.
목적 탐토Th세포각아군비례재궤양성결장염(UC)발병중적변화급의의.방법 안조2007년제남표준수집UC환자20례,대조조16례.이용세포내세포인자염색화사색형광항체류식세포술대장점막급외주혈림파세포작표형분석,비교각조장점막고유층단개핵세포(cLPMC)화외주혈단개핵세포(PBMC)중Th1、Th2、Th17세포비례적개변.결과 UC조cLPMC중Th17세포비례교대조조승고,분별위3.75%(6.93%)화1.25%(3.70%),재PBMC중위1.40%(2.15%)화0.70%(0.33%),량조간차이균유통계학의의(P치균<0.05).UC환자CLPMC중Th17세포비례여질병평분정정상관(r=0.34,P<0.05).UC조화대조조Th1、Th2세포재cLPMC화PBMC중적비례균차이무통즙학의의(P>0.05),차불동질병활동도환자간차이역무통계학의의(P치균>0.05).결론 재Th세포각아군중,Th17세포시개도UC장도국부화외주면역응답적우세세포,가능성위UC치료적유효파점.
Objective To analyze the changes of proportion of Th1/Th2/Th17 cells in colonic mucosa and peripheral blood in pathogenesis of ulcerative colitis (UC) and its significance. Methods Twenty patients with UC were enrolled according to the criterion established in Jinan in 2007 and 16 patients were served as controls. The phenotypes of the colonic mucosa and peripheral blood were analysis by using flow cytometry. The variation of proportion of Th1/Th2/Th17 in colonic lamina propria mononuclear cells (cLPMCs) and peripheral blood mononuclear cells (PBMCs) were compared between two groups. Results The proportion of Th17 cell in cLPMCs was increased in UC group compared with controls [3.75% (6.93%)vs. 1.25 (3.70%), P<0.05], whereas it was 1.40%(2.15%) in UC group and 0.70%(0.33%) in controls in PBMCs with significant difference (P<0.05). The proportion of Th17 cell in cLPMCs was positively correlated with clinical parameters of the disease (r=0.34, P<0.05). No difference was found in proportion of Th1 or Th2 cells in cLPMCs or PBMCs between UC group and controls. Conclusions Among three helper T cells, Th17 cell in UC patients is predominant both in colonic mucosa and peripheral blood, which can be used as an effective target for treatment.
