中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2008年
12期
1097-1100
,共4页
樊冤桥%何建桂%陈艺莉%陈钢%马虹%周训伦%胡心伟%彭红卫%赵斌
樊冤橋%何建桂%陳藝莉%陳鋼%馬虹%週訓倫%鬍心偉%彭紅衛%趙斌
번원교%하건계%진예리%진강%마홍%주훈륜%호심위%팽홍위%조빈
心肌梗死%利钠肽,脑%心室重构
心肌梗死%利鈉肽,腦%心室重構
심기경사%리납태,뇌%심실중구
Myocardial infarction%Natriuretic peptide,brain%Ventrieular remodeling
目的 探讨重组人脑利钠肽(rhBNP)对心肌梗死后大鼠心室重构及心功能的影响.方法 建立急性心肌梗死(AMI)雄性SD大鼠模型45只,随机分为AMI对照组、小剂量rhBNP治疗组、大剂最rhBNP治疗组,每组各15只.另设15只作假手术组.rhBNP治疗组经颈静脉输液管输注rhBNP,剂量分别为5μg/ks和15 μg/kg,1次/d,持续4周,假手术组及AMI对照组仅以等体积的生理盐水输注.4周后检测血液动力学及心功能参数,心脏标本检测左室重量及左室重量指数(LVMI)、心肌梗死面积,并检测血浆和心肌血管紧张素Ⅱ(Ang Ⅱ)水平.结果 AMI对照组左室重量、LVMI及心肌AngⅡ水平明显高于假手术组,而收缩压及左室内压最大上升和下降速率(±dp/dt)均明显低于假手术组(均P<0.01).大、小剂量rhBNP治疗组左室重量及LVMI、心肌梗死面积和心肌AngⅡ水平均明显低于AMI对照组[左室重量:分别为(492.6±34.0)mg、(498.8±47.8)mg比(570.0±24.2)mg,P<0.01;LVMI:2.0±0.2、2 0±0.2比2.3±0.1,P<0.01;心肌梗死面积:(25.3±2.9)%、(31.4±3.0)%比(46.4±3.0)%,P<0.01;Ang Ⅱ水平:(881.3±62.7)pg/L、(1186.0±94.5)pg/L比(2436.7±280.3)pg/L,P<0.05],收缩压和±dp/dt也高于AMI对照组(P<0.01或P<0.05).结论 外源性的rhBNP连续应用能限制梗死面积、减轻AMI后心室重构、保护心功能.
目的 探討重組人腦利鈉肽(rhBNP)對心肌梗死後大鼠心室重構及心功能的影響.方法 建立急性心肌梗死(AMI)雄性SD大鼠模型45隻,隨機分為AMI對照組、小劑量rhBNP治療組、大劑最rhBNP治療組,每組各15隻.另設15隻作假手術組.rhBNP治療組經頸靜脈輸液管輸註rhBNP,劑量分彆為5μg/ks和15 μg/kg,1次/d,持續4週,假手術組及AMI對照組僅以等體積的生理鹽水輸註.4週後檢測血液動力學及心功能參數,心髒標本檢測左室重量及左室重量指數(LVMI)、心肌梗死麵積,併檢測血漿和心肌血管緊張素Ⅱ(Ang Ⅱ)水平.結果 AMI對照組左室重量、LVMI及心肌AngⅡ水平明顯高于假手術組,而收縮壓及左室內壓最大上升和下降速率(±dp/dt)均明顯低于假手術組(均P<0.01).大、小劑量rhBNP治療組左室重量及LVMI、心肌梗死麵積和心肌AngⅡ水平均明顯低于AMI對照組[左室重量:分彆為(492.6±34.0)mg、(498.8±47.8)mg比(570.0±24.2)mg,P<0.01;LVMI:2.0±0.2、2 0±0.2比2.3±0.1,P<0.01;心肌梗死麵積:(25.3±2.9)%、(31.4±3.0)%比(46.4±3.0)%,P<0.01;Ang Ⅱ水平:(881.3±62.7)pg/L、(1186.0±94.5)pg/L比(2436.7±280.3)pg/L,P<0.05],收縮壓和±dp/dt也高于AMI對照組(P<0.01或P<0.05).結論 外源性的rhBNP連續應用能限製梗死麵積、減輕AMI後心室重構、保護心功能.
목적 탐토중조인뇌리납태(rhBNP)대심기경사후대서심실중구급심공능적영향.방법 건립급성심기경사(AMI)웅성SD대서모형45지,수궤분위AMI대조조、소제량rhBNP치료조、대제최rhBNP치료조,매조각15지.령설15지작가수술조.rhBNP치료조경경정맥수액관수주rhBNP,제량분별위5μg/ks화15 μg/kg,1차/d,지속4주,가수술조급AMI대조조부이등체적적생리염수수주.4주후검측혈액동역학급심공능삼수,심장표본검측좌실중량급좌실중량지수(LVMI)、심기경사면적,병검측혈장화심기혈관긴장소Ⅱ(Ang Ⅱ)수평.결과 AMI대조조좌실중량、LVMI급심기AngⅡ수평명현고우가수술조,이수축압급좌실내압최대상승화하강속솔(±dp/dt)균명현저우가수술조(균P<0.01).대、소제량rhBNP치료조좌실중량급LVMI、심기경사면적화심기AngⅡ수평균명현저우AMI대조조[좌실중량:분별위(492.6±34.0)mg、(498.8±47.8)mg비(570.0±24.2)mg,P<0.01;LVMI:2.0±0.2、2 0±0.2비2.3±0.1,P<0.01;심기경사면적:(25.3±2.9)%、(31.4±3.0)%비(46.4±3.0)%,P<0.01;Ang Ⅱ수평:(881.3±62.7)pg/L、(1186.0±94.5)pg/L비(2436.7±280.3)pg/L,P<0.05],수축압화±dp/dt야고우AMI대조조(P<0.01혹P<0.05).결론 외원성적rhBNP련속응용능한제경사면적、감경AMI후심실중구、보호심공능.
Objeclive To assess the effects of rhBNP on left ventrieular(LV)remodeling in rats with acute myocardial infaretion(AMI).Methods AMI was induced by ligating coronary arteryin male Sprague Dawley rats.Two days after surgery,AMI rats received intravenous infusion of rhBNP(15 μg/kg or 5 μg/kg once daily,n=15 each)or saline(placebo control,n=15)through Jugular Vein.Sham-operated mrs(a=15)served as normal control. Four weeks later,hemodynamie measurements were performed,left ventrieular weight (LVW),ratio of left ventfieular weight to body weight(LVW/BW),left ventrieular diameter(LVD)and infaret size were determined.P1asnla angiotensin Ⅱ and myocardial angiotensin Ⅱ levels were also measured.Results Compared with sham-operated rats,LVW,LVW/BW,LVD and myocardial angiotensin Ⅱ level were significantly increased,while the LV systolic pressure(LVSP),±dp/dt were significantly reduced in saline treated AMI rats(all P<0.05).LVW/BW,MI size,LVD and myocardial angiotensin Ⅱ in rhBNP treated AMI rats were significantly lower[LVW:(492.6±34.0)mg,(498.8±47.8)mg,(570.0±24.2)mg,P<0.01;LVW/BW:2.0±0.2,2.0±0.2,2.3±0.1,P<0.01;LVD:(25.3±2.9)%,(31.4±3.0)%,(46.4±3.0)%,P<0.01;myocardial angiotensin Ⅱ:(881.3±62.7)pg/L,(1186.0±94.5)pg/L,(2436.7±280.3)pg/L,P<0.05],while LVSP and ± dp/dt in rhBNP treatment groups were significantly increased than saline treated AMI rats f P<0.05 or P<0.01).Conclusion RhBNP is effective in attenuating left ventficular Itemodeling after AMI in rats.
