中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2011年
8期
571-574
,共4页
脑缺血再灌注%电针%血管新生%大鼠
腦缺血再灌註%電針%血管新生%大鼠
뇌결혈재관주%전침%혈관신생%대서
Cerebral ischemia%Reperfusion%Electroacupuncture%Angiogenesis
目的 研究电针对局灶性脑缺血再灌注大鼠大脑皮质微血管密度的影响。方法 将40只SD大鼠按随机数字表法随机分为正常组(n=4)、假手术组(n=4)、模型组(n=16)和电针组(n=16)。正常组常规饲养，不作任何处理，假手术组大鼠于麻醉切开颈部皮肤钝性分离肌层后，仅分离颈总动脉及颈内动脉至翼腭动脉，模型组和电针组均采用线栓法制备大鼠大脑中动脉闭塞(MCAO)模型。模型组和电针组于栓塞30 min后再灌注，并根据再灌注的时间点各分1d、2d、4d、8d四个亚组，每个亚组4只大鼠。电针组于缺血再灌注1h开始进行电针治疗，取“百会”、“水沟”、“足三里”穴，选疏密波治疗，每日1次，每次30 min。观察各组大鼠局灶性脑缺血30 min再灌注1d、2d、4d、8d后的右侧大脑皮质微血管CD34蛋白的表达及微血管密度的动态变化。结果 模型组各亚组大鼠的大脑皮质的微血管密度(MVD)较正常组和假手术组均有所增加，尤以缺血再灌注4d后最为显著，差异有统计学意义(P＜0.05)；在相同时间点，电针组各亚组大鼠的MVD均高于模型组，以缺血再灌注4d后2组差异有统计学意义(P＜0.05)。结论 调控脑内微血管新生可能是针刺发挥脑保护作用的途径之一。
目的 研究電針對跼竈性腦缺血再灌註大鼠大腦皮質微血管密度的影響。方法 將40隻SD大鼠按隨機數字錶法隨機分為正常組(n=4)、假手術組(n=4)、模型組(n=16)和電針組(n=16)。正常組常規飼養，不作任何處理，假手術組大鼠于痳醉切開頸部皮膚鈍性分離肌層後，僅分離頸總動脈及頸內動脈至翼腭動脈，模型組和電針組均採用線栓法製備大鼠大腦中動脈閉塞(MCAO)模型。模型組和電針組于栓塞30 min後再灌註，併根據再灌註的時間點各分1d、2d、4d、8d四箇亞組，每箇亞組4隻大鼠。電針組于缺血再灌註1h開始進行電針治療，取“百會”、“水溝”、“足三裏”穴，選疏密波治療，每日1次，每次30 min。觀察各組大鼠跼竈性腦缺血30 min再灌註1d、2d、4d、8d後的右側大腦皮質微血管CD34蛋白的錶達及微血管密度的動態變化。結果 模型組各亞組大鼠的大腦皮質的微血管密度(MVD)較正常組和假手術組均有所增加，尤以缺血再灌註4d後最為顯著，差異有統計學意義(P＜0.05)；在相同時間點，電針組各亞組大鼠的MVD均高于模型組，以缺血再灌註4d後2組差異有統計學意義(P＜0.05)。結論 調控腦內微血管新生可能是針刺髮揮腦保護作用的途徑之一。
목적 연구전침대국조성뇌결혈재관주대서대뇌피질미혈관밀도적영향。방법 장40지SD대서안수궤수자표법수궤분위정상조(n=4)、가수술조(n=4)、모형조(n=16)화전침조(n=16)。정상조상규사양，불작임하처리，가수술조대서우마취절개경부피부둔성분리기층후，부분리경총동맥급경내동맥지익악동맥，모형조화전침조균채용선전법제비대서대뇌중동맥폐새(MCAO)모형。모형조화전침조우전새30 min후재관주，병근거재관주적시간점각분1d、2d、4d、8d사개아조，매개아조4지대서。전침조우결혈재관주1h개시진행전침치료，취“백회”、“수구”、“족삼리”혈，선소밀파치료，매일1차，매차30 min。관찰각조대서국조성뇌결혈30 min재관주1d、2d、4d、8d후적우측대뇌피질미혈관CD34단백적표체급미혈관밀도적동태변화。결과 모형조각아조대서적대뇌피질적미혈관밀도(MVD)교정상조화가수술조균유소증가，우이결혈재관주4d후최위현저，차이유통계학의의(P＜0.05)；재상동시간점，전침조각아조대서적MVD균고우모형조，이결혈재관주4d후2조차이유통계학의의(P＜0.05)。결론 조공뇌내미혈관신생가능시침자발휘뇌보호작용적도경지일。
Objective To study the effects of electroacupuncture (EA) on micro-vessel density (MVD) in the cerebral cortex after focal cerebral ischemia and reperfusion.Methods Forty Sprague-Dawley rats were randomly divided into a normal group ( n =4), a sham-operated group (n =4) , a model group (n = 16) and an EA group (n = 16). The middle cerebral artery was sutured to establish a model of middle cerebral artery occlusion (MCAO). The Baihui (DU20), Shuigou (DU26) and Zusanli (ST36) acupoints were stimulated with slow-fast wave current for 30 min daily. Streptavidin-biotin-peroxidase complex was used to detect the expression of CD34 and MVD changes in the rats' right cerebral cortex on the 1st, 2nd, 4th and 8th day.Results Compared with the normal group and the sham-operated group, MVD in the ischemic cortex of all subgroups of the model group had increased. It peaked on the 4th day and then gradually declined through the 8th day. MVD in all the subgroups of the EA group increased significantly, and more than in the model group at every time point, but the difference between the 2 groups was significant only on the 4th day.Conclusions Regulating angiogenesis in ischemic brain tissue is one of the ways EA can protect the brain.
