中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
5期
334-339
,共6页
缺血再灌注%强化训练%胶质纤维酸性蛋白%碱性成纤维细胞生长因子%大鼠
缺血再灌註%彊化訓練%膠質纖維痠性蛋白%堿性成纖維細胞生長因子%大鼠
결혈재관주%강화훈련%효질섬유산성단백%감성성섬유세포생장인자%대서
Cerebral ischemia%Reperfusion%Training%Glial fibrillary acidic protein%Basic fibroblast growth factor%Swimming
目的 探讨强化训练对脑缺血再灌注大鼠梗死周围区胶质纤维酸性蛋白(GFAP)和碱性成纤维细胞生长因子(bFGF)表达的影响.方法 成年健康年雄性Wistar大鼠286只,体重250 ～300 g,清洁级,线栓法制作大脑中动脉缺血2h后再灌注3、7和14d大鼠模型,将造模成功的180只大鼠纳入研究,按随机数字表法分为运动训练Ⅰ组、运动训练Ⅱ组、运动训练Ⅲ组、运动训练Ⅳ组及对照组和假手术组,每组30只.运动训练Ⅰ组大鼠每天游泳1次,每次5 min；运动训练Ⅱ组大鼠每天游泳2次,每次5 min；运动训练Ⅲ组大鼠每天游泳1次,每次10 min；运动训练Ⅳ组大鼠每天游泳2次,每次10 min；对照组和假手术组大鼠不做任何训练.应用Bederson评分评价动物的神经功能恢复情况,氯化三苯基四氮唑(TTC)染色观察脑梗死体积,免疫组织化学法检测梗死周围区GFAP和bFGF的表达.结果 对照组大鼠各项指标与同一时间点假手术组比较,差异均有统计学意义(P＜0.01)；造模后第7和14天,各运动训练组大鼠Bederson 评分明显低于同一时间点的对照组(P＜0.05)；造模后3、7和14 d,各运动训练组大鼠脑梗死体积、GFAP 和bFGF阳性表达与同一时间点对照组比较,差异具有统计学意义(P＜0.01)；且运动训练Ⅳ组梗死灶体积减小、GFAP和bFGF阳性细胞表达增加较其它运动训练组更明显(P＜0.01).结论 运动训练可通过调节脑缺血再灌注后GFAP及bFGF的表达,促进神经细胞结构和功能重塑修复,且强化运动训练的效果更为显著.
目的 探討彊化訓練對腦缺血再灌註大鼠梗死週圍區膠質纖維痠性蛋白(GFAP)和堿性成纖維細胞生長因子(bFGF)錶達的影響.方法 成年健康年雄性Wistar大鼠286隻,體重250 ～300 g,清潔級,線栓法製作大腦中動脈缺血2h後再灌註3、7和14d大鼠模型,將造模成功的180隻大鼠納入研究,按隨機數字錶法分為運動訓練Ⅰ組、運動訓練Ⅱ組、運動訓練Ⅲ組、運動訓練Ⅳ組及對照組和假手術組,每組30隻.運動訓練Ⅰ組大鼠每天遊泳1次,每次5 min；運動訓練Ⅱ組大鼠每天遊泳2次,每次5 min；運動訓練Ⅲ組大鼠每天遊泳1次,每次10 min；運動訓練Ⅳ組大鼠每天遊泳2次,每次10 min；對照組和假手術組大鼠不做任何訓練.應用Bederson評分評價動物的神經功能恢複情況,氯化三苯基四氮唑(TTC)染色觀察腦梗死體積,免疫組織化學法檢測梗死週圍區GFAP和bFGF的錶達.結果 對照組大鼠各項指標與同一時間點假手術組比較,差異均有統計學意義(P＜0.01)；造模後第7和14天,各運動訓練組大鼠Bederson 評分明顯低于同一時間點的對照組(P＜0.05)；造模後3、7和14 d,各運動訓練組大鼠腦梗死體積、GFAP 和bFGF暘性錶達與同一時間點對照組比較,差異具有統計學意義(P＜0.01)；且運動訓練Ⅳ組梗死竈體積減小、GFAP和bFGF暘性細胞錶達增加較其它運動訓練組更明顯(P＜0.01).結論 運動訓練可通過調節腦缺血再灌註後GFAP及bFGF的錶達,促進神經細胞結構和功能重塑脩複,且彊化運動訓練的效果更為顯著.
목적 탐토강화훈련대뇌결혈재관주대서경사주위구효질섬유산성단백(GFAP)화감성성섬유세포생장인자(bFGF)표체적영향.방법 성년건강년웅성Wistar대서286지,체중250 ～300 g,청길급,선전법제작대뇌중동맥결혈2h후재관주3、7화14d대서모형,장조모성공적180지대서납입연구,안수궤수자표법분위운동훈련Ⅰ조、운동훈련Ⅱ조、운동훈련Ⅲ조、운동훈련Ⅳ조급대조조화가수술조,매조30지.운동훈련Ⅰ조대서매천유영1차,매차5 min；운동훈련Ⅱ조대서매천유영2차,매차5 min；운동훈련Ⅲ조대서매천유영1차,매차10 min；운동훈련Ⅳ조대서매천유영2차,매차10 min；대조조화가수술조대서불주임하훈련.응용Bederson평분평개동물적신경공능회복정황,록화삼분기사담서(TTC)염색관찰뇌경사체적,면역조직화학법검측경사주위구GFAP화bFGF적표체.결과 대조조대서각항지표여동일시간점가수술조비교,차이균유통계학의의(P＜0.01)；조모후제7화14천,각운동훈련조대서Bederson 평분명현저우동일시간점적대조조(P＜0.05)；조모후3、7화14 d,각운동훈련조대서뇌경사체적、GFAP 화bFGF양성표체여동일시간점대조조비교,차이구유통계학의의(P＜0.01)；차운동훈련Ⅳ조경사조체적감소、GFAP화bFGF양성세포표체증가교기타운동훈련조경명현(P＜0.01).결론 운동훈련가통과조절뇌결혈재관주후GFAP급bFGF적표체,촉진신경세포결구화공능중소수복,차강화운동훈련적효과경위현저.
Objective To explore the effects of different swimming training intensities on the expression of glial fibrillary acidic protein (GFAP) and basic fibroblast growth factor (bFGF) around a cerebral infarct. Methods The intraluminal thread method was applied to establish left middle cerebral artery occlusion (MCAO) for 2 h in 180 rats.They were then reperfused for 3,7 and 14 days.The rats were divided into four groups ( Ⅰ -Ⅳ) according to the intensity of the swimming training they were required to do,plus a control group and a sham operation group.The rats in training group Ⅰ swam 5 min,once daily; group Ⅱ trained for 5 min twice daily; group Ⅲ swam for 10 min once daily and group Ⅳ trained for 10 min twice daily.Neurological function was evaluated using Bederson's test,the cerebral infarction volume was calculated by tripeny tetrazolium chloride (TTC) staining.The expression of GFAP and bFGF around the infract were detected using immunohistochemistry. Results The average Bederson scores of the exercise training groups on the 7th and 14th days were significantly lower than that of the control group.The infarction volumes of each exercise training group were significantly lower than that of the control group,and cells positive for GFAP and bFGF in all training groups were significantly more numerous on the 3th,7th and 14th day after MCAO,especially in training group Ⅳ. Conclusion Intensive exercise can adjust the expression of GFAP and bFGF and promote the repair of brain damage after cerebral ischemia and reperfusion.
