CAJ | 학술논문

目的探讨垂体瘤转化基因(PTTG)在胶质瘤的诊断及恶性程度和预后判断中的临床意义.方法采用半定量逆转录聚合酶链反应法(RT-PCR)和蛋白免疫印迹法(Western-blot)对65例胶质瘤患者进行PTTG mRNA和PTTG蛋白检测.结果 (1)65例胶质瘤组织中PTTG蛋白和PTTG mRNA表达全部呈阳性.(2)计算分组的标本PTTG mRNA和与β-actin mRNA灰度比值:Ⅰ级和Ⅲ、Ⅳ级之间差别有统计学意义(P<0.01),Ⅲ、Ⅳ级之间PTTG mRNA表达均高于Ⅰ级;Ⅱ级和Ⅲ、Ⅳ级之间差别有统计学意义(P<0.01),Ⅲ、Ⅳ级之间PTTG mRNA表达均高于Ⅱ级.计算分组的标本PTTG蛋白和与β-actin吸光度比值:四组之间任意两两比较,差别均有统计学意义(P<0.01).(3)相关性分析:PTTG蛋白和PTTG mRNA表达水平与胶质瘤病理分级均呈正相关性(P<0.001).结论 (1)PTFG的mRNA和蛋白的表达与胶质瘤病理分级呈正相关.检测FTTG的mRNA和(或)蛋白的表达,可以作为胶质瘤诊断、判断预后的一个可靠指标.(2)检测PTTG的mRNA的表达,从分子病理方面将可以弥补传统病理的局限性,在脑肿瘤的诊断、治疗和预后评估中将发挥重大的作用.
목적 탐토수체류전화기인(PTTG)재효질류적진단급악성정도화예후판단중적림상의의.방법 채용반정량역전록취합매련반응법(RT-PCR)화단백면역인적법(Western-blot)대65례효질류환자진행PTTG mRNA화PTTG단백검측.결과 (1)65례효질류조직중PTTG단백화PTTG mRNA표체전부정양성.(2)계산분조적표본PTTG mRNA화여β-actin mRNA회도비치:Ⅰ급화Ⅲ、Ⅳ급지간차별유통계학의의(P<0.01),Ⅲ、Ⅳ급지간PTTG mRNA표체균고우Ⅰ급;Ⅱ급화Ⅲ、Ⅳ급지간차별유통계학의의(P<0.01),Ⅲ、Ⅳ급지간PTTG mRNA표체균고우Ⅱ급.계산분조적표본PTTG단백화여β-actin흡광도비치:사조지간임의량량비교,차별균유통계학의의(P<0.01).(3)상관성분석:PTTG단백화PTTG mRNA표체수평여효질류병리분급균정정상관성(P<0.001).결론 (1)PTFG적mRNA화단백적표체여효질류병리분급정정상관.검측FTTG적mRNA화(혹)단백적표체,가이작위효질류진단、판단예후적일개가고지표.(2)검측PTTG적mRNA적표체,종분자병리방면장가이미보전통병리적국한성,재뇌종류적진단、치료화예후평고중장발휘중대적작용.
Objective To explore the expression of PTTG in human glioma cell of the patients with neurogliocytoma and to see whether the PTTG's expression was associated with the differentiation and pathologic grading. It was expected that this study could reveal some essential qualities of the glioma and provide potential application for judging the malignant grades and biological treatment. Method The expression of PTTG and PTTG mRNA was detected in 65 cases of human glioma by hemi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and Western-blot analysis respectively. Results (1)In human glioma samples, the positive rate of PTTG mRNA and PTTG expression was 100%. (2)The gray degree ratios of PTTG mRNA belt to β-actin mRNA belt varied accordingly to different pathologic grades. There was significant difference between grade Ⅰ and grade Ⅲ or gradeⅣ(P<0.01). The same significance was also found between grade Ⅱ and grade Ⅲ or grade Ⅳ(P<0.01). The expressions of PTTG mRNA in grade Ⅲ or grade Ⅳ were significantly more than those of grade Ⅰ or grade Ⅱ. There was significant difference in the Reinhoit Zahl of PTTG belt to β-actin belt among groups (all P<0.01). (3)Analyses of correlation: The positive correlation existed between the expressions of PTTG or PTTG mRNA and the pathologic grading of human glioma(P<0.001). Conclusions (1) Detecting PTTG in human glioma can be used in diagnosing, judging the malignancy degree and the prognosis. (2) Detecting PTrG mRNA as molecular pathology in human glioma would retrieve the limitations of traditional tissue pathology. It will be an important cell marker that would act as more substantial role in future study.