CAJ | 학술논문

目的研究结直肠癌组织CDX2及p21~(WAF1)蛋白表达,探讨其在结直肠癌发生发展中的病理意义.在此基础上研究CDX2及p21~(WAF1)蛋白表达与中医虚证的相关性.方法以酒泉市人民医院的结直癌手术切除标本45例为研究对象,收集病理资料进行辨证分型.采用免疫组化EliVision法检测癌组织、癌旁组织中CDX2、及p21~(WAF1)的表达.结果 CDX2、p21~(WAF1)在结直肠癌组织中的阳性率分别为86.7%、35.6%.结直肠癌组织中CDX2与p21~(WAF1)表达无相关性.p21~(WAF1)蛋白表达在虚证组(11.76%)明显低于实证组(41.67%)和虚实夹杂证组(56.25%),其差异有统计学意义(x~2=7.385,P=0.025).CDX2蛋白表达在虚证、实证、虚实夹杂组间差别无统计学意义.结论 CDX2及p21~(WAF1)阳性率在痛及切缘组织中有显著性差异,二是因可能与结肠痛发生发展相关.CDX2与p21~(WAF1)之间无相关性.p21~(WAF1)表达降低提示结直肠癌患者预后不良.p21~(WAF1)表达在虚证组中明显低于实证、虚实夹杂证组.
목적 연구결직장암조직CDX2급p21~(WAF1)단백표체,탐토기재결직장암발생발전중적병리의의.재차기출상연구CDX2급p21~(WAF1)단백표체여중의허증적상관성.방법 이주천시인민의원적결직암수술절제표본45례위연구대상,수집병리자료진행변증분형.채용면역조화EliVision법검측암조직、암방조직중CDX2、급p21~(WAF1)적표체.결과 CDX2、p21~(WAF1)재결직장암조직중적양성솔분별위86.7%、35.6%.결직장암조직중CDX2여p21~(WAF1)표체무상관성.p21~(WAF1)단백표체재허증조(11.76%)명현저우실증조(41.67%)화허실협잡증조(56.25%),기차이유통계학의의(x~2=7.385,P=0.025).CDX2단백표체재허증、실증、허실협잡조간차별무통계학의의.결론 CDX2급p21~(WAF1)양성솔재통급절연조직중유현저성차이,이시인가능여결장통발생발전상관.CDX2여p21~(WAF1)지간무상관성.p21~(WAF1)표체강저제시결직장암환자예후불량.p21~(WAF1)표체재허증조중명현저우실증、허실협잡증조.
Objective To study the expression of CDX2 and p21~(WAF1) protein in colorectal cancer and its correlation with the progression of colorectal cancer., and to explore the eorrelativity of CDX2 and p21~(WAF1) genes expression with TCM deficiency Syndrome. Methods Immunohistochemical method was used to detect the expressions of CDX2 and p21~(WAF1) in45 patients of colorectal carcinoma tissues and peri-cancerous tissues, who received the treatment at the People's Hospital of Jiuquan City between April, 2000 and August, 2005. According to TCM syndrome differentiation, the patients were divided into sthenia syndrome group and asthenia syndrome group and sthenia-ashenia syndrome group. Analyze the Correlation between CDX2 and p21~(WAF1) expression and TCM syndrome. Results The positive rate of CDX2 and p21~(WAF1) was 86.7% and 35.6% respectively in colorectal carcinoma tissues. There was no correlation between CDX2 and p21~(WAF1) in colorectal cancer tissues. The positive rate of p21~(WAF1) was different in three groups, which was much lower in the asthenia syndrome group (11.76%) than the sthenia syndrome group (41.67%)and sthenia-asthenia syndrome group (56.25%). Conclusion CDX2 and p21~(WAF1) positive rate was different in cancer tissue and resection margin. The two genes may be associated with colorectal cancer development. CDX2 was not correlated with p21~(WAF1). The lower expression of p21~(WAF1) indicates poor prognosis. The positive rate of p21~(WAF1) was different between deficiency syndrome and excess syndrome, p21~(WAF1) is probably a marker to determine the asthenia and sthenia syndrome. Asthenia syndrome indicates a poor prognosis.