CAJ | 학술논문

目的观察双氢麦角碱对血管性痴呆(VD)小鼠海马组织乙酰胆碱代谢变化的影响.方法采用反复脑缺血-再灌注法制备VD模型;通过跳台试验和水迷宫试验测试小鼠的学习记忆成绩;采用免疫组化和原位杂交技术观测海马胆碱乙酰转移酶 (ChAT)及其mRNA表达的变化,并测定海马胆碱酯酶 (AchE)的活性变化.同时,以双氢麦角碱作为干预药物,观测其对以上指标的影响.结果双氢麦角碱显著改善了VD小鼠的学习、记忆成绩[(24.13±4.59)s,(125.35±32.45)s;(184.08±44.74)s,(79.57±29.08)s; (68.32±9.26)s,(143.29±17.39)s; (57.05±5.02)s,(162.36±10.61)s; P <0.01].同时,模型组小鼠海马ChAT(0.0856±0.0089)及其mRNA(0.0780±0.0122)表达较假手术组[(0.1226±0.0149),(0.1286±0.0085)]显著下降(P <0.01),其AchE的活性(14.53±0.86)也显著低于假手术组(17.15±1.02)(P <0.01).药物组小鼠海马AchE活性(16.67±0.71)、ChAT(0.1152±0.0095)及其mRNA(0.1199±0.0090)表达有明显改善(P <0.05).结论海马胆碱能系统损伤参与了VD的形成,双氢麦角碱可能通过提高海马组织ChAT及其mRNA和AchE的活性,从而改善胆碱能系统的功能.
목적 관찰쌍경맥각감대혈관성치태(VD)소서해마조직을선담감대사변화적영향.방법 채용반복뇌결혈-재관주법제비VD모형;통과도태시험화수미궁시험측시소서적학습기억성적;채용면역조화화원위잡교기술관측해마담감을선전이매 (ChAT)급기mRNA표체적변화,병측정해마담감지매 (AchE)적활성변화.동시,이쌍경맥각감작위간예약물,관측기대이상지표적영향.결과 쌍경맥각감현저개선료VD소서적학습、기억성적[(24.13±4.59)s,(125.35±32.45)s;(184.08±44.74)s,(79.57±29.08)s; (68.32±9.26)s,(143.29±17.39)s; (57.05±5.02)s,(162.36±10.61)s; P <0.01].동시,모형조소서해마ChAT(0.0856±0.0089)급기mRNA(0.0780±0.0122)표체교가수술조[(0.1226±0.0149),(0.1286±0.0085)]현저하강(P <0.01),기AchE적활성(14.53±0.86)야현저저우가수술조(17.15±1.02)(P <0.01).약물조소서해마AchE활성(16.67±0.71)、ChAT(0.1152±0.0095)급기mRNA(0.1199±0.0090)표체유명현개선(P <0.05).결론 해마담감능계통손상삼여료VD적형성,쌍경맥각감가능통과제고해마조직ChAT급기mRNA화AchE적활성,종이개선담감능계통적공능.
Objective To study the effect of dihydroergocriptine on the metabolic changes of acetylcholine in hippocampus of mice with vascular dementia. Methods The mice model with VD were established by ischemia-reperfusion method;and the behavior abnormalities were investigated by step-down test and water-maze test.The changes of ChAT were studied by immunohistochemistry and in situ hybridization technique. The activities of AchE in hippocampus was detected.Furthermore,the effect of dihydroergocriptine on the above indexes was investigated. Results The grades of learning and memory of VD mice were obviously improved by dihydroergocriptine[(24.13±4.59)s vs (125.35±32.45)s; (184.08±44.74)s vs (79.57±29.08)s; (68.32±9.26)s vs (143.29±17.39)s; (57.05±5.02)s vs (162.36±10.61)s; P <0.01]. In model group,the expression of ChAT (0.0856±0.0089) and its mRNA (0.0780±0.0122) was lower than which (0.1226±0.0149,0.1286±0.0085) in shame group (P <0.01),respectively. The activities of AchE (14.53±0.86) decreased significantly (P <0.01). In drug-treated group,the changes above mentioned were improved remarkably. Conclusions The abnormalities of cholinergic system in hippocampus might be involved into participating the formation of VD. Dihydroergocriptine could enhance the function of cholinergic system through promoting the expression of ChAT and its mRNA and increasing the activities of AchE.