中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2011年
6期
444-447
,共4页
牛嫣阳%余佩武%唐波%石彦%郝迎学
牛嫣暘%餘珮武%唐波%石彥%郝迎學
우언양%여패무%당파%석언%학영학
胃肿瘤%Ezrin%聚合酶链反应%蛋白质印迹法
胃腫瘤%Ezrin%聚閤酶鏈反應%蛋白質印跡法
위종류%Ezrin%취합매련반응%단백질인적법
Gastric neoplasms%Ezrin%Polymerase chain reaction%Western blot
目的 探讨Ezrin在胃癌发生及发展过程中的作用.方法 收集2008年6月至2009年5月第三军医大学西南医院全军普通外科中心收治的60例胃癌患者的胃癌组织及正常胃黏膜组织标本,分别采用RT-PCR和Western blot法检测标本中Ezrin mRNA及蛋白的表达情况,分析Ezrin与患者性别、年龄、肿瘤分化程度、病理分期、浸润深度及淋巴结转移间的关系.采用t检验、x2检验、Spearman等级相关检验进行统计学分析.结果 60例正常胃黏膜组织中有33例(55％) Ezrin mRNA表达水平升高,45例(75％)Ezrin蛋白表达水平升高；60例胃癌组织中有21例(35％) Ezrin mRNA表达水平升高,22例(37％) Ezrin蛋白表达水平升高.Ezrin mRNA及蛋白在正常胃黏膜组织中的表达水平分别为1.30±0.04和3.57±0.45,在胃癌组织中的表达水平分别为0.53±0.36和0.96±0.18,两者比较,差异有统计学意义(t=5.309,22.617,P＜0.05).胃癌组织中Ezrin mRNA及蛋白表达水平呈正相关(r=0.602,P＜0.05).Ezrin mRNA和蛋白表达在胃癌不同病理分期、不同浸润深度、有无淋巴结转移方面差异有统计学意义(x2=6.41,6.49,4.62;5.40,8.87,4.12,P＜0.05),而在性别、年龄和分化程度方面差异无统计学意义(x2=0.50,0.07,1.07；0.01,1.16,1.96,P＞0.05).结论 胃癌组织中Ezrin表达水平降低,这可能是胃癌的发生、发展及转移的机制之一.
目的 探討Ezrin在胃癌髮生及髮展過程中的作用.方法 收集2008年6月至2009年5月第三軍醫大學西南醫院全軍普通外科中心收治的60例胃癌患者的胃癌組織及正常胃黏膜組織標本,分彆採用RT-PCR和Western blot法檢測標本中Ezrin mRNA及蛋白的錶達情況,分析Ezrin與患者性彆、年齡、腫瘤分化程度、病理分期、浸潤深度及淋巴結轉移間的關繫.採用t檢驗、x2檢驗、Spearman等級相關檢驗進行統計學分析.結果 60例正常胃黏膜組織中有33例(55％) Ezrin mRNA錶達水平升高,45例(75％)Ezrin蛋白錶達水平升高；60例胃癌組織中有21例(35％) Ezrin mRNA錶達水平升高,22例(37％) Ezrin蛋白錶達水平升高.Ezrin mRNA及蛋白在正常胃黏膜組織中的錶達水平分彆為1.30±0.04和3.57±0.45,在胃癌組織中的錶達水平分彆為0.53±0.36和0.96±0.18,兩者比較,差異有統計學意義(t=5.309,22.617,P＜0.05).胃癌組織中Ezrin mRNA及蛋白錶達水平呈正相關(r=0.602,P＜0.05).Ezrin mRNA和蛋白錶達在胃癌不同病理分期、不同浸潤深度、有無淋巴結轉移方麵差異有統計學意義(x2=6.41,6.49,4.62;5.40,8.87,4.12,P＜0.05),而在性彆、年齡和分化程度方麵差異無統計學意義(x2=0.50,0.07,1.07；0.01,1.16,1.96,P＞0.05).結論 胃癌組織中Ezrin錶達水平降低,這可能是胃癌的髮生、髮展及轉移的機製之一.
목적 탐토Ezrin재위암발생급발전과정중적작용.방법 수집2008년6월지2009년5월제삼군의대학서남의원전군보통외과중심수치적60례위암환자적위암조직급정상위점막조직표본,분별채용RT-PCR화Western blot법검측표본중Ezrin mRNA급단백적표체정황,분석Ezrin여환자성별、년령、종류분화정도、병리분기、침윤심도급림파결전이간적관계.채용t검험、x2검험、Spearman등급상관검험진행통계학분석.결과 60례정상위점막조직중유33례(55％) Ezrin mRNA표체수평승고,45례(75％)Ezrin단백표체수평승고；60례위암조직중유21례(35％) Ezrin mRNA표체수평승고,22례(37％) Ezrin단백표체수평승고.Ezrin mRNA급단백재정상위점막조직중적표체수평분별위1.30±0.04화3.57±0.45,재위암조직중적표체수평분별위0.53±0.36화0.96±0.18,량자비교,차이유통계학의의(t=5.309,22.617,P＜0.05).위암조직중Ezrin mRNA급단백표체수평정정상관(r=0.602,P＜0.05).Ezrin mRNA화단백표체재위암불동병리분기、불동침윤심도、유무림파결전이방면차이유통계학의의(x2=6.41,6.49,4.62;5.40,8.87,4.12,P＜0.05),이재성별、년령화분화정도방면차이무통계학의의(x2=0.50,0.07,1.07；0.01,1.16,1.96,P＞0.05).결론 위암조직중Ezrin표체수평강저,저가능시위암적발생、발전급전이적궤제지일.
Objective To investigate the expression of Ezrin in gastric cancer tissues and its clinical significance.Methods Gastric cancer tissues and adjacent normal gastric tissues from 60 patients with gastric cancer were collected from June 2008 to May 2009 at the Southwest Hospital.The mRNA and protein expressions of Ezrin were detected by using the reverse transcription polymerase chain reaction and western blot.The relationship between Ezrin and the gender and age of patients,and tumor differentiation,pathological staging,depth of invasion and lymph node metastasis was analyzed.All data were analyzed using the t test,chi-square test and Spearman rank correlation.Results The Ezrin mRNA expression level was increased in 33 (55％) cases of adjacent normal gastric tissues and 21 (35％) cases of gastric cancer tissues; the Ezrin protein expression level was increased in 45 (75％) cases of adjacent normal gastric tissues and 22 (37％) cases of gastric cancer tissues.The mRNA and protein expressions of Ezrin in the normal adjacent gastric tissues were 1.30 ± 0.04 and 3.57 ± 0.45,respectively,which were significantly higher than 0.53 ± 0.36 and 0.96 ± 0.18 in the gastric cancer tissues ( t =5.309,22.617,P ＜ 0.05 ).The mRNA expression of Ezrin was positively correlated with the protein expression of Ezrin (r =0.602,P ＜ 0.05 ).The mRNA and protein expressions of Ezrin were related to the pathological stages,depth of invasion and state of lymph node metastasis (x2 =6.41,6.49,4.62; 5.40,8.87,4.12,P ＜ 0.05),but not to the gender,age and tumor differentiation (x2 =0.50,0.07,1.07 ; 0.01,1.16,1.96,P ＞ 0.05).Conclusion The mRNA and protein expressions of Ezrin are significantly decreased in the gastric cancer tissue,which might be responsible for genesis,development and metastasis of gastric cancer.
