中华医学杂志(英文版)
中華醫學雜誌(英文版)
중화의학잡지(영문판)
CHINESE MEDICAL JOURNAL
2001年
6期
588-591
,共4页
王纯巍%陆星华%徐彤%陈原稼%温小恒%钱家鸣%刘国仰%戴理%高春生
王純巍%陸星華%徐彤%陳原稼%溫小恆%錢傢鳴%劉國仰%戴理%高春生
왕순외%륙성화%서동%진원가%온소항%전가명%류국앙%대리%고춘생
Vater壶腹%胰腺肿瘤%染色体作图%遗传标记%染色体缺失
Vater壺腹%胰腺腫瘤%染色體作圖%遺傳標記%染色體缺失
Vater호복%이선종류%염색체작도%유전표기%염색체결실
Vater's ampulla%pancreatic neoplasms%chromosome mapping%genetic marker%chromosome deletion%polymorphism
目的进一步限定壶腹周围肿瘤染色体9p21区域缺失范围。
方法选择染色体9p21区域5个微卫星多态性标记,通过聚合酶链反应、聚丙烯酰胺凝胶电泳和银染法,检测35例壶腹周围肿瘤组织及其外周血杂合性丢失(LOH)状况。
结果 50%(4/8)胰腺癌有至少一个微卫星位点的LOH,其中D9S974(37.5%)和D9S942(28.6%)丢失频率较高,并且有连续性丢失现象。62.5%(5/8)壶腹癌在部分或全部位点出现LOH,其中D9S942(42.9%)丢失频率最高,其次为IFNA(37.5%)和D9S171(37.5%)。14.2%(1/7)胰岛素瘤有一个位点LOH。
结论壶腹周围肿瘤染色体9p21最小共同缺失区位于D9S974和D9S942位点之间,距离小于15kb,其中可能存在一个新的涉及该肿瘤发生的相关抑癌基因。
目的進一步限定壺腹週圍腫瘤染色體9p21區域缺失範圍。
方法選擇染色體9p21區域5箇微衛星多態性標記,通過聚閤酶鏈反應、聚丙烯酰胺凝膠電泳和銀染法,檢測35例壺腹週圍腫瘤組織及其外週血雜閤性丟失(LOH)狀況。
結果 50%(4/8)胰腺癌有至少一箇微衛星位點的LOH,其中D9S974(37.5%)和D9S942(28.6%)丟失頻率較高,併且有連續性丟失現象。62.5%(5/8)壺腹癌在部分或全部位點齣現LOH,其中D9S942(42.9%)丟失頻率最高,其次為IFNA(37.5%)和D9S171(37.5%)。14.2%(1/7)胰島素瘤有一箇位點LOH。
結論壺腹週圍腫瘤染色體9p21最小共同缺失區位于D9S974和D9S942位點之間,距離小于15kb,其中可能存在一箇新的涉及該腫瘤髮生的相關抑癌基因。
목적진일보한정호복주위종류염색체9p21구역결실범위。
방법선택염색체9p21구역5개미위성다태성표기,통과취합매련반응、취병희선알응효전영화은염법,검측35례호복주위종류조직급기외주혈잡합성주실(LOH)상황。
결과 50%(4/8)이선암유지소일개미위성위점적LOH,기중D9S974(37.5%)화D9S942(28.6%)주실빈솔교고,병차유련속성주실현상。62.5%(5/8)호복암재부분혹전부위점출현LOH,기중D9S942(42.9%)주실빈솔최고,기차위IFNA(37.5%)화D9S171(37.5%)。14.2%(1/7)이도소류유일개위점LOH。
결론호복주위종류염색체9p21최소공동결실구위우D9S974화D9S942위점지간,거리소우15kb,기중가능존재일개신적섭급해종류발생적상관억암기인。
Objective To further define the extent of chromosome 9p21 deletion in periampullary neoplasms.
Methods The loss of heterozygosity at 5 microsatellite polymorphic markers on chromosome 9p21 was detected by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis (PAGE) and silver staining in 35 specimens of periampullary neoplasms and their matching blood samples.
Results Fifty percent (4/8) of pancreatic cancer cases showed the loss of heterozygosity at one or more microsatellite loci, with the more frequent sites of D9S974 (37.5%) and D9S942 (28.6%), and some showing consecutive allelic loss. Sixty-two point five percent (5/8) of ampullary carcinoma cases showed loss of heterozygosity at one or more of the loci, frequent site of loss being D9S942 (42.9%) and the next most frequent being IFNA (37.5%) and D9S171 (37.5%). Loss of one locus was observed in 14.2% (1/7) of insulinoma.
Conclusion The minimal common region of chromosome deletion in periampullary neoplasms is defined between the D9S974 and D9S942 loci within a 15?kb interval in 9p21, suggesting the involvement of a novel tumor suppressor gene in their carcinogenesis.