CAJ | 학술논문

目的探讨大鼠局灶性脑缺血后神经功能恢复及海马病理形态学改变,评价依达拉奉的干预作用.方法 126只雄性SD大鼠随机分为3组:假手术组(A组,6只),生理盐水组(B组,MCAO后6h、12h、24h、48h、72h、5d、7d7时点,各6只),依达拉奉处理组(C组,同B组),术后即刻予以依达拉奉干预.行神经功能缺损评分测定；TTC染色观察脑梗死体积改变；用HE染色方法观察病理形态学改变.结果术后进行神经缺损评分发现,由于麻醉和手术创伤的影响,假手术组术后出现6～24h神经功能减退.与假手术组相比,在6～24h时间段盐水组神经功能下降明显(P＜0.05).依达拉奉组神经功能明显好于盐水组(P＜0.05).术后7d盐水组和依达拉奉组神经功能基本恢复.同时,在依达拉奉组和盐水组中,缺血24h脑梗死体积最大；与盐水组相比,术后6～24h依达拉奉组脑梗死体积明显减小(P＜0.05).HE染色显示术后6h在脑缺血区神经元细胞无明显改变,6h后缺血区脑组织逐渐出现肿胀与坏死；在依达拉奉组,脑水肿和神经元坏死病理损害明显较轻,在假手术组,脑组织无明显改变.结论依达拉奉具有改善神经功能缺损、缩小脑梗死体积和减轻缺血性病理损害程度的作用；研究还提示依达拉奉对缺血性脑卒中早期神经功能恢复具有十分重要的临床意义.
목적 탐토대서국조성뇌결혈후신경공능회복급해마병리형태학개변,평개의체랍봉적간예작용.방법 126지웅성SD대서수궤분위3조:가수술조(A조,6지),생리염수조(B조,MCAO후6h、12h、24h、48h、72h、5d、7d7시점,각6지),의체랍봉처리조(C조,동B조),술후즉각여이의체랍봉간예.행신경공능결손평분측정；TTC염색관찰뇌경사체적개변；용HE염색방법관찰병리형태학개변.결과 술후진행신경결손평분발현,유우마취화수술창상적영향,가수술조술후출현6～24h신경공능감퇴.여가수술조상비,재6～24h시간단염수조신경공능하강명현(P＜0.05).의체랍봉조신경공능명현호우염수조(P＜0.05).술후7d염수조화의체랍봉조신경공능기본회복.동시,재의체랍봉조화염수조중,결혈24h뇌경사체적최대；여염수조상비,술후6～24h의체랍봉조뇌경사체적명현감소(P＜0.05).HE염색현시술후6h재뇌결혈구신경원세포무명현개변,6h후결혈구뇌조직축점출현종창여배사；재의체랍봉조,뇌수종화신경원배사병리손해명현교경,재가수술조,뇌조직무명현개변.결론 의체랍봉구유개선신경공능결손、축소뇌경사체적화감경결혈성병리손해정도적작용；연구환제시의체랍봉대결혈성뇌졸중조기신경공능회복구유십분중요적림상의의.
Objective To investigate functiontal recovery and pathologic changes of hippocampus after focal cerebral ischemic injury in rats,and to evaluate the intervention effects of Edaravone.Methods 126 Sprague-Dawley male rats were randomly divided into three groups as shame-operated group(n =6,A),physiological saline group including 6hours、12hours 、24hours 、48hours、72hours、5days and 7days after MCAO (6 sub-groups,n =6 for each,B),edaravone-treated groups including the same time points as physiological saline groups(6 sub-groups,n =6 for each,C),edaravone intervention after operation.Model after the establishment,all the rats were tested for the neurologic impairment scores using Berderson Score methods at each time point.The volume of cerebral infarction was observed by TTC staining,and the pathologic changes were observed by HE staining.Results The neurological deficits were observed after operation.As the consequences of anesthesia and operation injury,the neurological function was decreased at 24 h after operation in shame-operated group.Compared with the shame-operated group,the neurological function in the physiological saline groups were decreased during the 6 h to 24 h(P ＜ 0.05).The damage of neurological function of the edaravone-treated groups were lighter than the physiological saline groups(P ＜0.05).At 7 d,the neurological function of the physiological saline groups and edaravone-treated groups almost recovered.Meanwhile,the infarct volume in the edaravone treat groups and the physiological saline groups reached their peaks at 24 hours post ischemia.Compared with the physiological saline groups,the infarct volume in the edaravone treat groups were decreased manifestly during 6 ～ 24 h after cerebral ischemia (P ＜0.05).The HE stain of the physiological saline groups indicated that the shape of neurons around the ischemic region had no obvious changes at 6 h.At6 h post ischemia,brain tissues around the ischemic focus were obviously swollen and necrotic.The pathological damages such as brain edema and neuron necrosis were ameliorated significantly in the edaravone treat groups.In the shame-operated groups,there were no significant changes in the brain tissue.Conclusion Edaravone can improve neurological impairment,reduce the volume of cerebral infarction and ischemic pathological lesion,and early interference of edaravone is of very important clinical significance for treatment of ischemic apoplexy.