CAJ | 학술논문

利用LC／MS／MS测定地沟油中胆固醇含量。研究过程中采用MRM—IDA—EPI的增强子离子扫描模式对样品进行分析，得到数据谱库检索，根据匹配度确证化合物的真伪，排除假阳性的可能，并且实现一次进样同时得到胆固醇的定量、定性结果。实验结果表明，该方法的最低定量限为50ng／mL，通过添加不同基质得到回收率为82％～93％。从结果中可看出与地沟油相比新植物油中胆固醇含量很低，所以若利用胆固醇作为地沟油的一个衡量指标，将为地沟油的确证提供有效依据。
이용LC／MS／MS측정지구유중담고순함량。연구과정중채용MRM—IDA—EPI적증강자리자소묘모식대양품진행분석，득도수거보고검색，근거필배도학증화합물적진위，배제가양성적가능，병차실현일차진양동시득도담고순적정량、정성결과。실험결과표명，해방법적최저정량한위50ng／mL，통과첨가불동기질득도회수솔위82％～93％。종결과중가간출여지구유상비신식물유중담고순함량흔저，소이약이용담고순작위지구유적일개형량지표，장위지구유적학증제공유효의거。
Cholesterol content was detected with LC/MS/MS method for identification of hogwash oil, and set up EPI （ enhance product ion） MS library. LC/MS/MS limit of quantification （LOQ） based on a signal/noise ratio of 10, was estimated to be for 50ng/mL. The MRM-IDA-EPI has shown the quantification and confirmation analysis in one injection, and the overall average recovery in cholesterol spiked oil as 82% ~93%. Compared with the hogwash oil,new oil was in a low quantity, so this method is well used for detection the cholesterol in the hogwash oil.