CAJ | 학술논문

采用不经过富集培养直接将土壤稀释涂布于营养物浓度较低的1／10LB固体培养基上，常温培养，然后转接至加淀粉的1／10LB固体培养基，用碘液初步检测淀粉酶分泌情况的方法，从土壤中分离出了5株能分泌淀粉酶的细菌，分别命名为K-1、K-2、K-3、K-4、K-5。并对其不同pH值、温度条件下的生长情况及分解淀粉产生还原糖的情况进行了初步研究。结果表明，菌株K-2具有产耐酸碱淀粉酶活性，在pH410范围内均可分解：定粉产生还原糖；K-4具有产低温淀粉酶活性，4℃能分解淀粉；K-5具有产高温耐碱淀粉酶活性。研究结果表明，采用低营养物浓度能从土壤中获得丰富多样的淀粉酶分泌细菌，为从自然环境中获取更丰富多样的微生物资源提供了基础参老．
채용불경과부집배양직접장토양희석도포우영양물농도교저적1／10LB고체배양기상，상온배양，연후전접지가정분적1／10LB고체배양기，용전액초보검측정분매분비정황적방법，종토양중분리출료5주능분비정분매적세균，분별명명위K-1、K-2、K-3、K-4、K-5。병대기불동pH치、온도조건하적생장정황급분해정분산생환원당적정황진행료초보연구。결과표명，균주K-2구유산내산감정분매활성，재pH410범위내균가분해：정분산생환원당；K-4구유산저온정분매활성，4℃능분해정분；K-5구유산고온내감정분매활성。연구결과표명，채용저영양물농도능종토양중획득봉부다양적정분매분비세균，위종자연배경중획취경봉부다양적미생물자원제공료기출삼로．
In the experiment, diluted soil samples were directly plated onto culture mediums with low concentration of nutrients （1/10 diluted LB solid culture medium） and cultivated at room temperature instead of enrichment culture, then it was plated onto 1/10 diluted LB solid culture medium with starch addition, and the secretion of amylase was determined by adding iodine solution to the surroundings of bacterial colonies to observe if it became blue, and 5 amylase-secreting bacteria strains were finally isolated and named as K-l, K-2, K-3, K-4., and K-5. The effects of different pH value and different temperature on their growth and their amylase hydrolysis status were investigated. The results showed that strain K-2 had acid ＆ alkali-resisting amylase activity and it could achieve amylase hydrolysis in wide range ofpH （pH4- 10）, and strain K-4 had activity at low temperature （it could achieve amylase hydrolysis at 4 ℃）, and strain K-5 had alkali-resisting amylase activity at high temperature. The results of our study suggested that various amylase-secreting bacteria could be obtained from soil by culture mediums with low concentration of nutrients, which provided experimental base for obtaining more microbial resources form natural environment.