CAJ | 학술논문

采用高效液相色谱法对玉米赤霉烯酮阳性样品进行定值,将阳性样品制备为不同毒素含量的验证样品,对胶体金免疫层析法的准确性、重复性、稳定性、最低检出限、误差范围进行了验证试验,并且完成了对实际样品的检测,同时采用酶联免疫法（ELISA）进行对比,试验结果为：最低检出限为60μg/kg,其检测结果与酶联免疫法基本一致,方法具有操作简便、快速、灵敏度高、特异性强、稳定性好、分析结果易于判定的特点,适用于样品的现场快速检测。
채용고효액상색보법대옥미적매희동양성양품진행정치,장양성양품제비위불동독소함량적험증양품,대효체금면역층석법적준학성、중복성、은정성、최저검출한、오차범위진행료험증시험,병차완성료대실제양품적검측,동시채용매련면역법（ELISA）진행대비,시험결과위：최저검출한위60μg/kg,기검측결과여매련면역법기본일치,방법구유조작간편、쾌속、령민도고、특이성강、은정성호、분석결과역우판정적특점,괄용우양품적현장쾌속검측。
The corn sample with zearalenone positive was set by HPLC.The sample was prepared as several different samples with different poison content.The accuracy,repetitiveness,stability,minimum detection limit and error range of the colloidal gold immunochromatography was evaluated by test,contrasted with enzyme linked immunosorbent assay.The result was that the minimun detection limit was 60μg/kg in colloidal gold immunochromatography,which was similar to enzyme linked immunosorbent assay.The colloidal gold immunochromatography had the advantages of easy and quick to operate,with high sensitivity,specificity and stability,and easy to determine.It is suitable for quick test on spot.