CAJ | 학술논문

[目的]对桉树基因组中葡萄糖-6-磷酸脱氢酶（G6PDH）进行全基因组分析与进化研究。[方法]利用巨桉（Eucalyptus grandsis）全基因组数据,采用BLAST等生物信息学软件分析桉树G6PDH的基因特性、蛋白序列、系统进化树以及启动子特征。[结果]桉树基因组内存在6个G6PDH基因,其编码蛋白中,1个为胞质型G6PDH,5个为质体型G6PDH,而且桉树G6PDH均含有保守基序motif1、motif2、motif3、motif7、motif9和motif11。此外,桉树G6PDH启动子序列中均存在TATA框、增强子与涉及光应答、激素应答、胁迫应答等调控元件。[结论]可为下一步研究桉树G6PDH家族基因的分子功能提供参考。
[목적]대안수기인조중포도당-6-린산탈경매（G6PDH）진행전기인조분석여진화연구。[방법]이용거안（Eucalyptus grandsis）전기인조수거,채용BLAST등생물신식학연건분석안수G6PDH적기인특성、단백서렬、계통진화수이급계동자특정。[결과]안수기인조내존재6개G6PDH기인,기편마단백중,1개위포질형G6PDH,5개위질체형G6PDH,이차안수G6PDH균함유보수기서motif1、motif2、motif3、motif7、motif9화motif11。차외,안수G6PDH계동자서렬중균존재TATA광、증강자여섭급광응답、격소응답、협박응답등조공원건。[결론]가위하일보연구안수G6PDH가족기인적분자공능제공삼고。
[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase（G6PDH） and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database.[Result] Six G6PDH genes,including one cytomic type and five plastids,were detected in the E.grandsis genome.All the G6PDHs have conserved motifs of motif 1,motif 2,motif 3,motif 7,motif 9 and motif 11.Furthermore,promoter sequences of all E.grandsis G6PDH contain TATA box,enhancer,light-responsive,hormone-responsive and stress-responsive regulatory elements.[Conclusion] This study provided reference for the further revealing molecular function of E.grandsis G6PDH gene family