泰州职业技术学院学报
泰州職業技術學院學報
태주직업기술학원학보
JOURNAL OF TAIZHOU POLYTECHNICAL INSTITUTE
2011年
3期
78-81
,共4页
谢献胜%刘孟%王小波%李建基%彭大新
謝獻勝%劉孟%王小波%李建基%彭大新
사헌성%류맹%왕소파%리건기%팽대신
幽门螺杆菌%感染%蒙古沙土鼠%疾病模型
幽門螺桿菌%感染%矇古沙土鼠%疾病模型
유문라간균%감염%몽고사토서%질병모형
helicobacter pylori%infections%mongolian gerbi%disease mode
利用蒙古沙土鼠建立幽门螺杆菌(Helicobacter pylori,Hp)NCTC 11637株胃内感染模型。感染方法有两种,方法Ⅰ是沙土鼠禁食18h后用50%酒精0.5mL预处理,6h后接种Hp,间隔12h、24h分别再次接种。方法Ⅱ的接种方法同方法Ⅰ,但接种后每日口服雷尼替丁(5m g/kg体重)。胃内接种H p后,于第7天、15天、35天、45天分别用Hp抗原酶联免疫诊断试剂盒、细菌培养和胃组织切片的方法观察沙土鼠胃内细菌感染情况和胃病理组织学变化。结果表明,给沙土鼠胃内灌服酒精以损伤胃黏膜并每日口服雷尼替丁,在人工接种35天后,胃内见有多量Hp生长,胃组织病变与Hp自然感染的病例相似。
利用矇古沙土鼠建立幽門螺桿菌(Helicobacter pylori,Hp)NCTC 11637株胃內感染模型。感染方法有兩種,方法Ⅰ是沙土鼠禁食18h後用50%酒精0.5mL預處理,6h後接種Hp,間隔12h、24h分彆再次接種。方法Ⅱ的接種方法同方法Ⅰ,但接種後每日口服雷尼替丁(5m g/kg體重)。胃內接種H p後,于第7天、15天、35天、45天分彆用Hp抗原酶聯免疫診斷試劑盒、細菌培養和胃組織切片的方法觀察沙土鼠胃內細菌感染情況和胃病理組織學變化。結果錶明,給沙土鼠胃內灌服酒精以損傷胃黏膜併每日口服雷尼替丁,在人工接種35天後,胃內見有多量Hp生長,胃組織病變與Hp自然感染的病例相似。
이용몽고사토서건립유문라간균(Helicobacter pylori,Hp)NCTC 11637주위내감염모형。감염방법유량충,방법Ⅰ시사토서금식18h후용50%주정0.5mL예처리,6h후접충Hp,간격12h、24h분별재차접충。방법Ⅱ적접충방법동방법Ⅰ,단접충후매일구복뢰니체정(5m g/kg체중)。위내접충H p후,우제7천、15천、35천、45천분별용Hp항원매련면역진단시제합、세균배양화위조직절편적방법관찰사토서위내세균감염정황화위병리조직학변화。결과표명,급사토서위내관복주정이손상위점막병매일구복뢰니체정,재인공접충35천후,위내견유다량Hp생장,위조직병변여Hp자연감염적병례상사。
Two methods were used to establish models of Helicobacter pylori(NCTC11637) infection in the Mongolian gerbil stomach..The method Ⅰwas that the Mongolian gerbils were forbidden to eat for18 hours,then feed 0.5mL of 50% alcohol and inoculated Helicobacter pylori three times at a 6h-,12h-,and 24h-interval.The method Ⅱ was that it was treated as same as the above method,but taken Ranitidine orally everyday,with 5mg/kg body weight.On the 7th,15th,35th,and the 45th day after inoculation,the infection and pathological changes in gastric mucosa were observed by Hp antigen diagnostic ELISA kit,bacterial culture,and histological section.The results showed that there were much Helicobacter pylori in the stomach in 35 days after artificial inoculated Helicobacter pylori,combining the gastric mucous membrane damaged by alcohol with Ranitidine by oral administration per day,and the pathological changes were the same with that in natural infected cases.