CAJ | 학술논문

为了明确2010年食源性疾病监测中分离的肠道沙门菌O：4（B）菌群的PFGE分子型别，探讨其多态性及其与分子流行病学关系，我们将分离获得的29株O：4（B）血清型沙门菌进一步鉴定培养，挑取单个菌落增菌，供试菌株基因组DNA用限制性内切酶xbaI消化酶切后进行脉冲场凝胶电泳分型，所得结果用Bionumerics5．1软件进行聚类分析。实验结果表明，根据电泳指纹图谱，可将29株肠道沙门菌O：4（B）菌群分为24个PFGE型别，菌株间的相似值在56.31％～100％之间，同一血清型别的沙门菌有多个PFGE型别。脉冲场凝胶电泳对O：4（B）群沙门菌有较高的分型能力，可有效的应用于食物中沙门菌溯源分析及分子流行病学研有。
위료명학2010년식원성질병감측중분리적장도사문균O：4（B）균군적PFGE분자형별，탐토기다태성급기여분자류행병학관계，아문장분리획득적29주O：4（B）혈청형사문균진일보감정배양，도취단개균락증균，공시균주기인조DNA용한제성내절매xbaI소화매절후진행맥충장응효전영분형，소득결과용Bionumerics5．1연건진행취류분석。실험결과표명，근거전영지문도보，가장29주장도사문균O：4（B）균군분위24개PFGE형별，균주간적상사치재56.31％～100％지간，동일혈청형별적사문균유다개PFGE형별。맥충장응효전영대O：4（B）군사문균유교고적분형능력，가유효적응용우식물중사문균소원분석급분자류행병학연유。
The objective of the present study was to document the polymorphism of PFGE molecular type of Salmonella enterica subsp, enterica serotype O：4（B） which isolated from food-borne diseases and to explore theirs relationship of molecular epidemiological. In this research, 29 Salmonella stains were identified and the single colony was picked and enriched. Then the total genomic DNA of the isolates was digested with restriction enzyme Xba I, and run pulsed-field gel electrophoresis （PFGE）. The DNA fingerprints of different stains were analyzed by Bionumerics5.1 software to perform clustering. The DNA fingerprints of 29 Salmonella were belonged to 24 PFGE pattern combinations. Similarity value of these strains was in the range of 56.31%-100%. One serotype Salmonella could be found several PFGE pattern combinations. The PFGE technology is a good method for molecular typing of Salmonella serotype O：4（B） and could be effectively apply on tracing the suspected source of food contamination and investigate the food-poisoning and also study the molecular epidemiological relationship of different Salmonella stains.