西北林学院学报
西北林學院學報
서북림학원학보
JOURNAL OF NORTHWEST FORESTRY UNIVERSITY
2010年
2期
108-112
,共5页
王启瑞%朱刚利%郑哲民%范钦%孙学刚
王啟瑞%硃剛利%鄭哲民%範欽%孫學剛
왕계서%주강리%정철민%범흠%손학강
缘蝽科%姬缘蝽亚科%蛛缘蝽亚科%酯酶同工酶%基因座%多态性
緣蝽科%姬緣蝽亞科%蛛緣蝽亞科%酯酶同工酶%基因座%多態性
연춘과%희연춘아과%주연춘아과%지매동공매%기인좌%다태성
Coreidae%Rhopaline%Alydinae%esterase isozyme%locus%polymorphism
应用聚丙烯酰胺凝胶电泳技术研究了秦岭4种缘蝽的EST同工酶.研究结果表明,姬缘蝽亚科2属间差异要大于属内各种间差异.控制开环缘蝽及点蜂缘蝽的EST位点分别为4和5个,而且乙酸萘酯为底物的酯酶除未发现杂合子而无法确定的以外,都是单体酶,不同个体间酶谱差异主要是同一位点来自父母本的2个等位基因的不同而造成的,雌雄差异可能由性连锁基因引起.点蜂缘蝽3个居群等位基因频率存在差异,还需要进一步试验研究.
應用聚丙烯酰胺凝膠電泳技術研究瞭秦嶺4種緣蝽的EST同工酶.研究結果錶明,姬緣蝽亞科2屬間差異要大于屬內各種間差異.控製開環緣蝽及點蜂緣蝽的EST位點分彆為4和5箇,而且乙痠萘酯為底物的酯酶除未髮現雜閤子而無法確定的以外,都是單體酶,不同箇體間酶譜差異主要是同一位點來自父母本的2箇等位基因的不同而造成的,雌雄差異可能由性連鎖基因引起.點蜂緣蝽3箇居群等位基因頻率存在差異,還需要進一步試驗研究.
응용취병희선알응효전영기술연구료진령4충연춘적EST동공매.연구결과표명,희연춘아과2속간차이요대우속내각충간차이.공제개배연춘급점봉연춘적EST위점분별위4화5개,이차을산내지위저물적지매제미발현잡합자이무법학정적이외,도시단체매,불동개체간매보차이주요시동일위점래자부모본적2개등위기인적불동이조성적,자웅차이가능유성련쇄기인인기.점봉연춘3개거군등위기인빈솔존재차이,환수요진일보시험연구.
This paper deals with the esterase isozymes of four species of subfamily Rhopaline and Alydinae(Hemiptera:Coreidae) in Qinling Mountainous region by the vertical slab polyacrylamide gel electrophoretic technique. The variances between the two genera of Rhopalinae are obvious than intraspecific differences. There are 4 and 5 loci resolved for Stictopleurus minutus and Riptortus pedestris, respectively. All the esterase present as monomeric enzyme except for these which were dominated by monomorphic loci. The isozyme polymorphism of individuals within a Coreidae species is results from the pair of different alleles at the same loci. And sex-linked gene may result in the difference between female and male within species. Differences in allic among three populations of R. pedestric appeared, but the data available is limited and calls for further research.