光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2010年
2期
508-511
,共4页
郭雪峰%岳永德%孟志芬%汤锋%王进%姚曦%荀航%孙嘏
郭雪峰%嶽永德%孟誌芬%湯鋒%王進%姚晞%荀航%孫嘏
곽설봉%악영덕%맹지분%탕봉%왕진%요희%순항%손하
竹叶提取物%羟自南基%清除率%IC_(50)值
竹葉提取物%羥自南基%清除率%IC_(50)值
죽협제취물%간자남기%청제솔%IC_(50)치
Bamboo lear extract%Hydroxyl free radical%Scavenging rate%IC_(50) value
进行了Fe~(2+)与邻二氮菲牛成红色配合物的吸收光谱,抗氧化剂TBHQ及竹叶提取物样品对清除羟自由基能力的研究.分光光度法测定抗氧化剂清除羟自由基能力的测定波长为509.1 nm.以IC_(50)值(清除率为50%时,抗氧化剂的浓度值)作为评价抗氧化剂清除羟自由基能力的指标,测得合成抗氧化剂和效果最好竹叶提取物样品IC_(50)值分别为0.040(TBHQ),0.378(M20),0.323(M40),0.334(M60),M20,M40,M60等竹叶提取物可以作为天然抗氧化剂进行开发.
進行瞭Fe~(2+)與鄰二氮菲牛成紅色配閤物的吸收光譜,抗氧化劑TBHQ及竹葉提取物樣品對清除羥自由基能力的研究.分光光度法測定抗氧化劑清除羥自由基能力的測定波長為509.1 nm.以IC_(50)值(清除率為50%時,抗氧化劑的濃度值)作為評價抗氧化劑清除羥自由基能力的指標,測得閤成抗氧化劑和效果最好竹葉提取物樣品IC_(50)值分彆為0.040(TBHQ),0.378(M20),0.323(M40),0.334(M60),M20,M40,M60等竹葉提取物可以作為天然抗氧化劑進行開髮.
진행료Fe~(2+)여린이담비우성홍색배합물적흡수광보,항양화제TBHQ급죽협제취물양품대청제간자유기능력적연구.분광광도법측정항양화제청제간자유기능력적측정파장위509.1 nm.이IC_(50)치(청제솔위50%시,항양화제적농도치)작위평개항양화제청제간자유기능력적지표,측득합성항양화제화효과최호죽협제취물양품IC_(50)치분별위0.040(TBHQ),0.378(M20),0.323(M40),0.334(M60),M20,M40,M60등죽협제취물가이작위천연항양화제진행개발.
By studying on absorption spectrum of red compound coming from the reaction system of Fe~(2+) and 1,10-phenanthroline and the capacity of antioxidant TBHQ and bamboo leaf extract for scavenging hydroxyl free radical,some results were drawn as follows:the determining wavelength of bamboo leaf extract for scavenging hydroxyl free radical by spectrophotometric method is 509.1 nm,and IC_(50)(the value of antioxidant concentration at scavenging half of hydroxyl free radical)was used as the index to evaluate scavenging capacity.The determined IC_(50) values were TBHQ(0.040),M20(0.378),M40(0.323),M60(0.334),and bamboo leaf extract could be used as natural antioxidant.
