CAJ | 학술논문

[目的]明确不同栽培年限人参根区土壤真菌、细菌和放线菌浓度变化情况.[方法]以吉林省多个产地人参根区土壤作为研究对象,采用稀释乎板法对真菌、细菌和放线菌进行分离和数量测定,真菌的分离用马丁培养基+孟加拉红+硫酸链霉素;细菌的分离用牛肉膏蛋白胨培养基;放线菌的分离用高氏1号培养基+重铬酸钾.真菌培养温度为25℃,培养时间为6～7 d;放线菌培养温度为28℃,培养时间为4～5 d;细菌培养温度为30℃,培养时间为2～4 d.根据不同培养基中生长出的菌落数统计真菌、细菌和放线菌数量,结合土壤样品的稀释倍数,按照公式:土壤微生物浓度(cfu/g)=(菌落平均数×稀释倍数)/每皿菌液加入量(ml),求得土壤中可培养真菌、细菌和放线菌浓度.[结果]不同地点采集的土壤样品的统计结果存在差异,但基本表现为随人参生长年限的增加,人参根区土壤中真菌浓度逐年升高,细菌和放线菌浓度逐年降低,且放线菌的变化不如细菌变化显著.[结论]土壤类型、种植模式及人参生长年限对农田土和林地土中3类微生物的浓度均有影响,但生长年限对人参根区土壤微生物的影响最显著.
[목적]명학불동재배년한인삼근구토양진균、세균화방선균농도변화정황.[방법]이길림성다개산지인삼근구토양작위연구대상,채용희석호판법대진균、세균화방선균진행분리화수량측정,진균적분리용마정배양기+맹가랍홍+류산련매소;세균적분리용우육고단백동배양기;방선균적분리용고씨1호배양기+중락산갑.진균배양온도위25℃,배양시간위6～7 d;방선균배양온도위28℃,배양시간위4～5 d;세균배양온도위30℃,배양시간위2～4 d.근거불동배양기중생장출적균락수통계진균、세균화방선균수량,결합토양양품적희석배수,안조공식:토양미생물농도(cfu/g)=(균락평균수×희석배수)/매명균액가입량(ml),구득토양중가배양진균、세균화방선균농도.[결과]불동지점채집적토양양품적통계결과존재차이,단기본표현위수인삼생장년한적증가,인삼근구토양중진균농도축년승고,세균화방선균농도축년강저,차방선균적변화불여세균변화현저.[결론]토양류형、충식모식급인삼생장년한대농전토화임지토중3류미생물적농도균유영향,단생장년한대인삼근구토양미생물적영향최현저.
[Objective]To see the dynamic of fungi,bacilli and actinomyces communities from root region of ginseng with different growing years.[Method]With ginseng root region soils from several sampling sites of Jilin Province as materials,concentrations of fungi,bacilli and antinomyces were evaluated by spread-plate method.[Result]Though there are differences on statistic data among soil samples,commonly with the increasing of growing years,concentration of fungi in ginseng root region increased,which were on the contrary for bacilli and antinomyces,and bacilli changed even more significant than antinomyces.[Conclusion]Concentrations of soil microorganisms can be influenced by soil type,planting mode and growing years simultaneously,but growing years influenced even more significantly.