国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2010年
1期
1-4
,共4页
沈轶%张婉玉%George C Y Chiou
瀋軼%張婉玉%George C Y Chiou
침질%장완옥%George C Y Chiou
naringenin%碘酸钠%激光%视网膜色素上皮细胞%年龄相关性黄斑变性
naringenin%碘痠鈉%激光%視網膜色素上皮細胞%年齡相關性黃斑變性
naringenin%전산납%격광%시망막색소상피세포%년령상관성황반변성
naringenin%sodium iodate%laser%retinal pigment epithelium%age-related macular degeneration
目的:研究naringenin滴眼对碘酸钠诱导的大鼠视网膜色素上皮(retinal pigment epithelium,RPE)变性以及对激光诱导的脉络膜新生血管(choroidal neovascularization,CNV)的作用.方法:10g/L naringenin滴眼液预先处理1wk(3次/d),1wk后予35mg/kg碘酸钠舌下静脉注射诱导大鼠RPE变性,在2wk和4wk末,视网膜电图(electroretinogram,ERG)测量C波.另预处理1wk(3次/d),在2wk和4wk末用荧光素血管造影(fluorescein angiography,FA)和荧光显微镜测量CNV面积.结果:碘酸钠注射后2wk,碘酸钠组ERG的C波下降至对照组的53%(P<0.01).而naringenin +碘酸钠组则无明显变化.4wk后,碘酸钠组下降至对照组的37%(P<0.01),而naringenin +碘酸钠组下降至对照组的57%(P<0.01).与碘酸钠组比较,naringenin +碘酸钠组控制了66%的C波下降(P<0.05).35mg/kg naringenin组FA测量的CNV面积2,4wk末分别是对照组的53%和49%(P<0.01).4wk后naringenin组荧光显微镜测量的CNV面积是对照组的47%(P<0.01).结论:10g/L naringenin可以显著保护碘酸钠诱导的RPE变性,也能减小CNV的形成.
目的:研究naringenin滴眼對碘痠鈉誘導的大鼠視網膜色素上皮(retinal pigment epithelium,RPE)變性以及對激光誘導的脈絡膜新生血管(choroidal neovascularization,CNV)的作用.方法:10g/L naringenin滴眼液預先處理1wk(3次/d),1wk後予35mg/kg碘痠鈉舌下靜脈註射誘導大鼠RPE變性,在2wk和4wk末,視網膜電圖(electroretinogram,ERG)測量C波.另預處理1wk(3次/d),在2wk和4wk末用熒光素血管造影(fluorescein angiography,FA)和熒光顯微鏡測量CNV麵積.結果:碘痠鈉註射後2wk,碘痠鈉組ERG的C波下降至對照組的53%(P<0.01).而naringenin +碘痠鈉組則無明顯變化.4wk後,碘痠鈉組下降至對照組的37%(P<0.01),而naringenin +碘痠鈉組下降至對照組的57%(P<0.01).與碘痠鈉組比較,naringenin +碘痠鈉組控製瞭66%的C波下降(P<0.05).35mg/kg naringenin組FA測量的CNV麵積2,4wk末分彆是對照組的53%和49%(P<0.01).4wk後naringenin組熒光顯微鏡測量的CNV麵積是對照組的47%(P<0.01).結論:10g/L naringenin可以顯著保護碘痠鈉誘導的RPE變性,也能減小CNV的形成.
목적:연구naringenin적안대전산납유도적대서시망막색소상피(retinal pigment epithelium,RPE)변성이급대격광유도적맥락막신생혈관(choroidal neovascularization,CNV)적작용.방법:10g/L naringenin적안액예선처리1wk(3차/d),1wk후여35mg/kg전산납설하정맥주사유도대서RPE변성,재2wk화4wk말,시망막전도(electroretinogram,ERG)측량C파.령예처리1wk(3차/d),재2wk화4wk말용형광소혈관조영(fluorescein angiography,FA)화형광현미경측량CNV면적.결과:전산납주사후2wk,전산납조ERG적C파하강지대조조적53%(P<0.01).이naringenin +전산납조칙무명현변화.4wk후,전산납조하강지대조조적37%(P<0.01),이naringenin +전산납조하강지대조조적57%(P<0.01).여전산납조비교,naringenin +전산납조공제료66%적C파하강(P<0.05).35mg/kg naringenin조FA측량적CNV면적2,4wk말분별시대조조적53%화49%(P<0.01).4wk후naringenin조형광현미경측량적CNV면적시대조조적47%(P<0.01).결론:10g/L naringenin가이현저보호전산납유도적RPE변성,야능감소CNV적형성.
AIM: To study the effects of naringenin eye drops on NaIO3-induced retinal pigment epithelium (RPE) degeneration and laser-induced choroidal neovascularization (CNV) in rat eyes.METHODS: The 35mg/kg NaIO3-induced RPE degeneration was prevented by 10g/L naringenin eye drops 3 times a day for 7 days in advance of NaIO3 injection, and then 2 to 4 weeks thereafter, RPE function was measured with C-wave of electroretinogram (ERG). The laser-induced CNV rats were treated with laser to break the Bruchs membrane and the CNV formation was prevented by 10g/L naringenin eye drops instilled 3 times a day for 2 to 4 weeks. The CNV formation was measured with fluorescein angiography (FA) and flat mount. RESULTS: Two weeks after NaIO3 injection, the amplitude of ERG C-wave fell markedly in NaIO3 group to 53% of normal group (P<0.01). No apparent difference was observed in naringenin+ NaIO3 group. Four weeks later, the NaIO3 group fell to 37% of normal group (P<0.01), while the naringenin+ NaIO3 group fell to only 57% of normal group (P<0.01). There is a 52% reversal of the ERG C-wave by naringenin as compared to NaIO3 treated group (P<0.05). Two weeks and four weeks after laser treatment, naringenin reduced the CNV formation to 53% and 49% of control group (100%) measured by FA (P<0.01). Four weeks after laser treatment, naringenin reduced the CNV formation by 47% as compared to control group measured with flat mount (P<0.01).CONCLUSION: Naringenin significantly protected RPE from NaIO3 induced degeneration and can also prevent CNV formation.
