时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
7期
1661-1662
,共2页
梁陈方%杨春旭%吴洪文%李锦燊
樑陳方%楊春旭%吳洪文%李錦燊
량진방%양춘욱%오홍문%리금신
高效液相色谱法%蝎龙酒%阿魏酸
高效液相色譜法%蝎龍酒%阿魏痠
고효액상색보법%갈룡주%아위산
Ferulic acid%Xielong jiu%Determination%HPLC
目的 建立测定蝎龙酒活性成分阿魏酸含量的高效液相色谱法.方法 采用Vp-ODS柱(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水 (用磷酸调pH值为2.5～3) (25∶75) 为流动相,流速为0.8 ml/min,柱温30 ℃,检测波长320 nm.结果 阿魏酸在3.66～36.64 mg·L-1浓度范围内,峰面积与浓度线性关系良好,相关系数r=0.999 8,加样平均回收率为100.47%(RSD=0.84%).结论 该法简便,准确,可用于蝎龙酒中阿魏酸的含量测定.
目的 建立測定蝎龍酒活性成分阿魏痠含量的高效液相色譜法.方法 採用Vp-ODS柱(250 mm×4.6 mm,5 μm)色譜柱,以乙腈-水 (用燐痠調pH值為2.5～3) (25∶75) 為流動相,流速為0.8 ml/min,柱溫30 ℃,檢測波長320 nm.結果 阿魏痠在3.66～36.64 mg·L-1濃度範圍內,峰麵積與濃度線性關繫良好,相關繫數r=0.999 8,加樣平均迴收率為100.47%(RSD=0.84%).結論 該法簡便,準確,可用于蝎龍酒中阿魏痠的含量測定.
목적 건립측정갈룡주활성성분아위산함량적고효액상색보법.방법 채용Vp-ODS주(250 mm×4.6 mm,5 μm)색보주,이을정-수 (용린산조pH치위2.5～3) (25∶75) 위류동상,류속위0.8 ml/min,주온30 ℃,검측파장320 nm.결과 아위산재3.66～36.64 mg·L-1농도범위내,봉면적여농도선성관계량호,상관계수r=0.999 8,가양평균회수솔위100.47%(RSD=0.84%).결론 해법간편,준학,가용우갈룡주중아위산적함량측정.
Objective To establish an HPLC method for determination of Ferulic acid content in Xielong jiu.Methods Samples were determined by RP-HPLC on a Vp-ODS column(250 mm×4.6 mm,5μm) with a mixture of acetonitrile-water(adjusted pH to 2.5～3 with phosphoric acid)(25:75) as mobile phase at the flow velocity of 0.8ml/min and UV detection at 320 nm wavelength.Results The calibration curve of Ferulic acid was linear from 3.66 to 36.64 mg/L (r=0.999 8,n=6),the average recovery and relative standard deviation were 100.47% and 0.84% (n=5) respectively.Conclusion The method is rapid,simple and accurate.It can be used for assay of Ferulic acid content in Xielong jiu.
