背景:中风安口服液是治疗脑血管疾病的新型中药制剂,主要成分为黄芪和水蛭,其中黄芪具有明显的益气活血作用,水蛭具有很强的抗血小板、抗血栓、缓解动脉痉挛和改善组织缺氧的作用.目的:观察中风安口服液对大鼠动脉血栓形成和对小鼠血液凝固及耐力的影响.设计:完全随机对照实验.单位:河北医科大学基础医学院药理教研室.材料:实验于2001-09/2002-04在河北医科大学药理研究室完成.药物影响血栓形成实验:第1组实验取Wistar大鼠40只,随机分为中风安3.0,6.0,12.0g/kg剂量组、阿司匹林0.3 g/kg组和对照组,每组8只.第2组实验取Wistar大鼠50只,随机分为中风安3.0,6.0 g/kg剂量组、脑血康3.0,6.0 g/kg剂量组和对照组,每组10只.小鼠凝血时间实验:取小鼠50只,随机分为中风安6.0,12.0,24.0 g/kg剂量组、阿司匹林0.3 g/kg组和对照组,每组10只.小鼠游泳耗竭时间测定:取小鼠90只,随机分为中风安6.0,24.0 g/kg剂量组;脑血康6.0,24.0 g/kg剂量组,苯丙胺0.2 g/kg组和对照组,每组15只.方法:药物影响血栓形成实验,第1组于术前24 h及1 h各组大鼠分别灌胃给予中风安(3,6,12g/kg),阿司匹林(0.3g/kg)和水.第2组大鼠术前24h及1 h分别灌胃给予中风安(3,6g/kg)脑血康(3,6 g/kg)和水.给药后腹腔注射氯胺酮50 mg/kg麻醉,采用线栓血栓法,测定各鼠血栓湿重,比较各组血栓形成的差异.小鼠凝血时间的测定,分别灌胃给予中风安(24.0,12.0,6.0 g/kg),阿司匹林(0.3 g/kg)和水,于灌胃后1 h用玻片法观察小鼠凝血时间.小鼠游泳耗竭时间的测定,灌胃给予中风安(6.0,24.0 g/kg)和脑血康(6.0,24.0 g/kg)及苯丙胺(0.2 g/kg,)和水.1次/d,连续灌胃5 d,于第5天给药后1 h测定小鼠游泳耗竭时间.计算各组小鼠游泳耗竭时间的平均值.主要观察指标:①各组大鼠血栓形成抑制率.②各组小鼠凝血时间.③各组小鼠游泳耗竭时间.结果:参加实验的90只大鼠和140只小鼠均进入结果分析.①血栓湿重:第1组实验:中风安口服液3.0,6.0,12.0 g/kg剂量组明显低于对照组[(24±4),(21±4),(16±6),(39±7)mg,(t=5.88～7.90,P＜0.01)];第2组实验:中风安口服液6.0 g/kg剂量组明显低于相同剂量脑血康组[(23.6±2.6),(30.0±4.1),(t=4.18,P＜0.01)],中风安口服液3.0 g/kg剂量组低于同剂量脑血康组[(30.6±2.1),(33.1±1.6)mg,(t=2.96,P＜0.05)].②凝血时间:中风安口服液24.0,12.0g/kg剂量组明显高于对照组[(222±66),(190±52),(116±26)s,(t=4.02,4.72,P＜0.01)].③游泳耗竭时间:中风安24.0,6.0g/kg剂量组明显高于对照组[(2 512±1 244),(899±403),(502±100)s,(t=3.70～6.24,P＜0.01)].结论:中风安口服液对大鼠动脉血栓和小鼠静脉血栓的形成均有明显的抑制作用,并可增强小鼠的耐力,具有抗疲劳作用.
揹景:中風安口服液是治療腦血管疾病的新型中藥製劑,主要成分為黃芪和水蛭,其中黃芪具有明顯的益氣活血作用,水蛭具有很彊的抗血小闆、抗血栓、緩解動脈痙攣和改善組織缺氧的作用.目的:觀察中風安口服液對大鼠動脈血栓形成和對小鼠血液凝固及耐力的影響.設計:完全隨機對照實驗.單位:河北醫科大學基礎醫學院藥理教研室.材料:實驗于2001-09/2002-04在河北醫科大學藥理研究室完成.藥物影響血栓形成實驗:第1組實驗取Wistar大鼠40隻,隨機分為中風安3.0,6.0,12.0g/kg劑量組、阿司匹林0.3 g/kg組和對照組,每組8隻.第2組實驗取Wistar大鼠50隻,隨機分為中風安3.0,6.0 g/kg劑量組、腦血康3.0,6.0 g/kg劑量組和對照組,每組10隻.小鼠凝血時間實驗:取小鼠50隻,隨機分為中風安6.0,12.0,24.0 g/kg劑量組、阿司匹林0.3 g/kg組和對照組,每組10隻.小鼠遊泳耗竭時間測定:取小鼠90隻,隨機分為中風安6.0,24.0 g/kg劑量組;腦血康6.0,24.0 g/kg劑量組,苯丙胺0.2 g/kg組和對照組,每組15隻.方法:藥物影響血栓形成實驗,第1組于術前24 h及1 h各組大鼠分彆灌胃給予中風安(3,6,12g/kg),阿司匹林(0.3g/kg)和水.第2組大鼠術前24h及1 h分彆灌胃給予中風安(3,6g/kg)腦血康(3,6 g/kg)和水.給藥後腹腔註射氯胺酮50 mg/kg痳醉,採用線栓血栓法,測定各鼠血栓濕重,比較各組血栓形成的差異.小鼠凝血時間的測定,分彆灌胃給予中風安(24.0,12.0,6.0 g/kg),阿司匹林(0.3 g/kg)和水,于灌胃後1 h用玻片法觀察小鼠凝血時間.小鼠遊泳耗竭時間的測定,灌胃給予中風安(6.0,24.0 g/kg)和腦血康(6.0,24.0 g/kg)及苯丙胺(0.2 g/kg,)和水.1次/d,連續灌胃5 d,于第5天給藥後1 h測定小鼠遊泳耗竭時間.計算各組小鼠遊泳耗竭時間的平均值.主要觀察指標:①各組大鼠血栓形成抑製率.②各組小鼠凝血時間.③各組小鼠遊泳耗竭時間.結果:參加實驗的90隻大鼠和140隻小鼠均進入結果分析.①血栓濕重:第1組實驗:中風安口服液3.0,6.0,12.0 g/kg劑量組明顯低于對照組[(24±4),(21±4),(16±6),(39±7)mg,(t=5.88～7.90,P＜0.01)];第2組實驗:中風安口服液6.0 g/kg劑量組明顯低于相同劑量腦血康組[(23.6±2.6),(30.0±4.1),(t=4.18,P＜0.01)],中風安口服液3.0 g/kg劑量組低于同劑量腦血康組[(30.6±2.1),(33.1±1.6)mg,(t=2.96,P＜0.05)].②凝血時間:中風安口服液24.0,12.0g/kg劑量組明顯高于對照組[(222±66),(190±52),(116±26)s,(t=4.02,4.72,P＜0.01)].③遊泳耗竭時間:中風安24.0,6.0g/kg劑量組明顯高于對照組[(2 512±1 244),(899±403),(502±100)s,(t=3.70～6.24,P＜0.01)].結論:中風安口服液對大鼠動脈血栓和小鼠靜脈血栓的形成均有明顯的抑製作用,併可增彊小鼠的耐力,具有抗疲勞作用.
배경:중풍안구복액시치료뇌혈관질병적신형중약제제,주요성분위황기화수질,기중황기구유명현적익기활혈작용,수질구유흔강적항혈소판、항혈전、완해동맥경련화개선조직결양적작용.목적:관찰중풍안구복액대대서동맥혈전형성화대소서혈액응고급내력적영향.설계:완전수궤대조실험.단위:하북의과대학기출의학원약리교연실.재료:실험우2001-09/2002-04재하북의과대학약리연구실완성.약물영향혈전형성실험:제1조실험취Wistar대서40지,수궤분위중풍안3.0,6.0,12.0g/kg제량조、아사필림0.3 g/kg조화대조조,매조8지.제2조실험취Wistar대서50지,수궤분위중풍안3.0,6.0 g/kg제량조、뇌혈강3.0,6.0 g/kg제량조화대조조,매조10지.소서응혈시간실험:취소서50지,수궤분위중풍안6.0,12.0,24.0 g/kg제량조、아사필림0.3 g/kg조화대조조,매조10지.소서유영모갈시간측정:취소서90지,수궤분위중풍안6.0,24.0 g/kg제량조;뇌혈강6.0,24.0 g/kg제량조,분병알0.2 g/kg조화대조조,매조15지.방법:약물영향혈전형성실험,제1조우술전24 h급1 h각조대서분별관위급여중풍안(3,6,12g/kg),아사필림(0.3g/kg)화수.제2조대서술전24h급1 h분별관위급여중풍안(3,6g/kg)뇌혈강(3,6 g/kg)화수.급약후복강주사록알동50 mg/kg마취,채용선전혈전법,측정각서혈전습중,비교각조혈전형성적차이.소서응혈시간적측정,분별관위급여중풍안(24.0,12.0,6.0 g/kg),아사필림(0.3 g/kg)화수,우관위후1 h용파편법관찰소서응혈시간.소서유영모갈시간적측정,관위급여중풍안(6.0,24.0 g/kg)화뇌혈강(6.0,24.0 g/kg)급분병알(0.2 g/kg,)화수.1차/d,련속관위5 d,우제5천급약후1 h측정소서유영모갈시간.계산각조소서유영모갈시간적평균치.주요관찰지표:①각조대서혈전형성억제솔.②각조소서응혈시간.③각조소서유영모갈시간.결과:삼가실험적90지대서화140지소서균진입결과분석.①혈전습중:제1조실험:중풍안구복액3.0,6.0,12.0 g/kg제량조명현저우대조조[(24±4),(21±4),(16±6),(39±7)mg,(t=5.88～7.90,P＜0.01)];제2조실험:중풍안구복액6.0 g/kg제량조명현저우상동제량뇌혈강조[(23.6±2.6),(30.0±4.1),(t=4.18,P＜0.01)],중풍안구복액3.0 g/kg제량조저우동제량뇌혈강조[(30.6±2.1),(33.1±1.6)mg,(t=2.96,P＜0.05)].②응혈시간:중풍안구복액24.0,12.0g/kg제량조명현고우대조조[(222±66),(190±52),(116±26)s,(t=4.02,4.72,P＜0.01)].③유영모갈시간:중풍안24.0,6.0g/kg제량조명현고우대조조[(2 512±1 244),(899±403),(502±100)s,(t=3.70～6.24,P＜0.01)].결론:중풍안구복액대대서동맥혈전화소서정맥혈전적형성균유명현적억제작용,병가증강소서적내력,구유항피노작용.
OBJECTIVE: Oral zhongfeng an liquid is a new dose form of traditional Chinese medicine for treating cerebrovascular diseases. Its main components are astragalus and hirudo, the former is of obvious effects of replenishing qi and activating blood, and the latter is of stronger effects of antiplatelet, antithrombosis and arteriospasm-reducing, as well as improving tissue anoxia.OBJECTIVE: To investigate the effects of oral zhongfeng an liquid on arterial thrombosis in rats, and blood coagulation and tolerance in mice.DESIGN: A completely randomized and controlled trial.SETTING: Pharmacological Division of Basic Medical College, Hebei Medical University.MATERIALS: The experiment was completed from September 2001 to April 2002 at the Pharmacological Division of Basic Medical College,Hebei Medical University. The experiment of effect of the drug on thrombosis in rats: In the first study, totally 40 Wistar rats were at random divided into five groups: zhongfeng an liquid 3.0, 6.0, 12.0 g/kg, aspirin 0.3 g/kg and control group, with 8 in each group. In the second study, totally 50 Wistar rats were also at random divided into five groups: zhongfeng an liquid 3.0 and 6.0 g/kg, naoxue kang 3.0 and 6.0 g/kg and control group,with 10 in each group. Clotting time study in mice: Totally 50 mice were randomly divided as zhongfeng an liquid 6.0, 12.0, 24.0 g/kg, aspirin 0.3 g/kg and control group, with 10 in each group. Measurement of swimming exhaustion time of mice: Totally 90 mice were randomly divided as zhongfeng an liquid 6.0 and 24.0 g/kg, naoxue kang 6.0 and 24.0 g/kg, benzedrine 0.2 g/kg and control group, with 15 in each group.METHODS: In the experiment of effect of the drug on thrombosis: For the first study, 24 hours and 1 hour before operation the rats in all groups were respectively by gavage given oral zhongfeng an liquid (3, 6, 12 g/kg), aspirin (0.3 g/kg), and water. For the second study, 24 hours and 1 hour before operation the rats were respectively by gavage given oral zhongfeng an liquid (3, 6 g/kg), naoxue kang (3, 6 g/kg) and water. After administration,ketamine 50 mg/kg was peritoneally given for anesthesia, silk ligature thrombosis was used, then the wet thrombus was weighed for comparison of difference in thrombosis among the groups. Measurement of clotting time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (24.0, 12.0, 6.0 g/kg), aspirin (0.3 g/kg) and water, one hour after administration the clotting time of mice was detected with the slide method. Measurement of swimming exhaustion time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (6.0, 24.0 g/kg), naoxue kang (6.0, 24.0 g/kg), Benzedrine (0.2 g/kg) and water, once a day for 5 days.On the fifth day 1 hour after administration, the swimming exhaustion time of mice was measured, the mean value of swimming exhaustion time of mice in each group was calculated.haustion time of mice in each group.RESULTS: All 90 rats and 140 mice involved entered into the result thrombus in rats of the oral zhongfeng an liquid 3.0, 6.0 and 12.0 g/kg groups were obviously lower than those in the naoxue kang groups of the same dose [(24±4), (21±4), (16±6), (39±7) mg, (t=5.88-7.90, P ＜ 0.01)]; in the second study, the wet quality of rats in the oral zhongfeng an liquid 6.0 g/kg group was obviously lower than that in the same dose naoxue kang group [(23.6±2.6), (30.0±4.1), (t=4.18, P ＜ 0.01)], the wet quality of mice in the oral zhongfeng an liquid 3.0 g/kg group was obviously lower than that in the same dose naoxue kang group [(30.6±2.1), (33.1±1.6) mg, (t=2.96,zhongfeng an liquid 24.0 and 12.0 g/kg groups were obviously higher than that in the control group [(222±66), (190±52), (116±26) s, (t=4.02, 4.72, P the oral zhongfeng an liquid 24.0 and 6.0 g/kg groups were obviously higher than that in the control group [(2 512±1 244), (899±403), (502±100) s,(t=3.70-6.24, P ＜ 0.01)].CONCLUSION: Oral zhongfeng an liquid was of obvious inhibition to arterial thrombosis of rats and venous thrombosis of mice, and could enhance the tolerance of mice with a role of antifatigue.
