CAJ | 학술논문

目的　探讨胎盘生长因子(PLGF)与妊娠高血压综合征（妊高征）发病的关系。方法用酶联免疫吸附法(ELISA)、免疫组织化学染色法和逆转录-聚合酶链反应法(RT-PCR)检测23例妊高征患者(妊高征组)、20例正常妊娠妇女(正常妊娠组)的外周血及其新生儿脐静脉血PLGF水平、胎盘蜕膜PLGF蛋白及其mRNA表达。结果　(1)妊高征组外周血PLGF水平［(112.7±63.8) μg/L］低于正常妊娠组［(200.3±140.9) μg/L］，两组比较，差异有显著性 (P<0.05)，且与新生儿体重和胎盘重量呈正相关，相关系数分别为0.4、0.6。(2)妊高征组合体滋养细胞、绒毛间质和蜕膜PLGF表达均显著低于正常妊娠组(P<0.01，P<0.01，P<0.05)，其合体滋养细胞PLGF表达水平与妊高征病情的轻、重有关。(3)妊高征组胎盘和蜕膜组织总PLGF mRNA表达均显著低于正常妊娠组(P<0.05，P<0.05)，尤其胎盘组织主要表现为PLGF-2 mRNA表达明显下降(P<0.01)。结论　PLGF的表达异常在妊高征发病中起着重要作用。
목적　탐토태반생장인자(PLGF)여임신고혈압종합정（임고정）발병적관계。방법용매련면역흡부법(ELISA)、면역조직화학염색법화역전록-취합매련반응법(RT-PCR)검측23례임고정환자(임고정조)、20례정상임신부녀(정상임신조)적외주혈급기신생인제정맥혈PLGF수평、태반세막PLGF단백급기mRNA표체。결과　(1)임고정조외주혈PLGF수평［(112.7±63.8) μg/L］저우정상임신조［(200.3±140.9) μg/L］，량조비교，차이유현저성 (P<0.05)，차여신생인체중화태반중량정정상관，상관계수분별위0.4、0.6。(2)임고정조합체자양세포、융모간질화세막PLGF표체균현저저우정상임신조(P<0.01，P<0.01，P<0.05)，기합체자양세포PLGF표체수평여임고정병정적경、중유관。(3)임고정조태반화세막조직총PLGF mRNA표체균현저저우정상임신조(P<0.05，P<0.05)，우기태반조직주요표현위PLGF-2 mRNA표체명현하강(P<0.01)。결론　PLGF적표체이상재임고정발병중기착중요작용。
Objective　To determine whether pregnancy induced hypertension syndrome (PIHs) is associated with placenta growth factor (PLGF). Methods　PLGF level in the maternal and umbilical venous serum collected from 23 PIHs patients and 20 normotensive controls was measured by enzyme linked immunoassay (ELISA), and expression of PLGF and its mRNA in the placenta and decidua from them was assessed by the immuno-histochemistry and reverse transcriptional-polymerase chain reaction (RT-PCR). Results　(1) PLGF levels in the maternal serum from PIHs ［(112.7±63.8) μg/L］ were significantly lower than those from normal pregnancies ［(200.3±140.9) μg/L］,(P<0.05), and they were correlated with infant birth weight and placental mass (r=0.4, r=0.6). (2) Expression of PLGF in sycytiotrophoblast and villous mesenchyme and decidua from PIHs significantly decreased respectively (P<0.01, P<0.01, P<0.05), and expression of PLGF in sycytiotrophoblast was associated with the severity of PIHs (P<0.05). (3) The transcription of total PLGF mRNA in the placenta and decidua from PIHs decreased significantly (P<0.05 and P<0.05 respectively). Particularly, the transcription of PLGF-2 mRNA decreased significantly in the placenta from PIHs. Conclusion　The abnormal expression of PLGF may play an important role in the pathogenesis of placental ischaemia hypoxia in PIHs.