CAJ | 학술논문

背景:脑震荡的机制未明,脑啡肽和多巴胺在其中的表达及意义不清.目的:探讨大鼠脑震荡脑组织中脑啡肽和多巴胺的表达及意义.设计:随机对照实验研究.地点和材料:实验地点:解放军军事医学科学院.健康Wistar雄性二级(清洁级)大鼠80只,军事医学科学院实验动物中心清洁级动物房饲养,水料任意,用于复制脑震荡动物模型.依致脑震荡所用砝码质量的不同随机分为对照组和50,100,200 g组.干预:实验大鼠于伤后1,3,7,14及30 d活杀,取脑组织,经免疫组化染色等技术,研究脑震荡发生发展中脑啡肽和多巴胺的变化规律.主要观察指标:①临床表现.②病理学改变.③脑啡肽和多巴胺表达的免疫组化染色结果.结果:100 g大鼠组出现典型的脑震荡临床表现,其病理改变为脑血管收缩与扩张、脑组织瘀血与水肿、神经元变性、凋亡与坏死.脑啡肽于伤后1 d表达增强,阳性部位见于大脑、小脑和海马区的血管内皮细胞浆中;于伤后7 d达高峰.14 d后表达逐渐减少,30 d仍高于正常水平.多巴胺于伤后7 d,在大脑、海马区、丘脑及小脑各部分血管内皮细胞、血管壁的表达明显增多,但各时间点无明显变化.结论:脑震荡后以血液循环障碍和实质细胞变性和坏死为主要病理改变.脑啡肽和多巴胺参与了脑震荡发生时脑损伤的病理生理过程,可能对血管损伤、血脑屏障和神经细胞变性、坏死起重要的调节作用.
배경:뇌진탕적궤제미명,뇌배태화다파알재기중적표체급의의불청.목적:탐토대서뇌진탕뇌조직중뇌배태화다파알적표체급의의.설계:수궤대조실험연구.지점화재료:실험지점:해방군군사의학과학원.건강Wistar웅성이급(청길급)대서80지,군사의학과학원실험동물중심청길급동물방사양,수료임의,용우복제뇌진탕동물모형.의치뇌진탕소용겁마질량적불동수궤분위대조조화50,100,200 g조.간예:실험대서우상후1,3,7,14급30 d활살,취뇌조직,경면역조화염색등기술,연구뇌진탕발생발전중뇌배태화다파알적변화규률.주요관찰지표:①림상표현.②병이학개변.③뇌배태화다파알표체적면역조화염색결과.결과:100 g대서조출현전형적뇌진탕림상표현,기병리개변위뇌혈관수축여확장、뇌조직어혈여수종、신경원변성、조망여배사.뇌배태우상후1 d표체증강,양성부위견우대뇌、소뇌화해마구적혈관내피세포장중;우상후7 d체고봉.14 d후표체축점감소,30 d잉고우정상수평.다파알우상후7 d,재대뇌、해마구、구뇌급소뇌각부분혈관내피세포、혈관벽적표체명현증다,단각시간점무명현변화.결론:뇌진탕후이혈액순배장애화실질세포변성화배사위주요병리개변.뇌배태화다파알삼여료뇌진탕발생시뇌손상적병리생리과정,가능대혈관손상、혈뇌병장화신경세포변성、배사기중요적조절작용.
BACKGROUND: The mechanisms of cerebral concussion have remained poorly understood, and so do the expression profiles of enkephalin and dopamine and their significance in this injury.OBJECTIVE: To investigate the expressions of enkephalin and dopamine and explore their significance in rat cerebral concussion tissue.DESIGN: A randomized controlled trial.SETTING and MATERIALS: This study was conducted in the Academy of Military Medical Sciences of PLA. Totally 80 male Wistar rats of clean grade, provided by the Animal Center of Academy of Military Medical Sciences and given free access to water and food, were used for preparing animal models of cerebral concussion. The rats were randomly divided into 4 groups according to the different cerebral impact for model establishment using counterweights, namely the control group, 50, 100 and 200 g groups.INTERVENTIONS: The rats were sacrificed on the day 1, 3, 7, 14 and 30 following brain concussion, respectively, and the brain tissue were taken for examination of the expressions of enkephalin and dopamine by means of immunohistochemical staining.MAIN OUTCOME MEASURES: The manifestations, pathological changes, and the result of immunohistochemical staining on enkephalin and dopamine were observed.RESULTS: Typical features of cerebral concussion were seen in rats of the 100 g group. The main pathological changes .included cerebral vascular constriction and dilation, hemostasis and edema of the cerebral tissue and neuronal degeneration and necrosis. Enkephalin expression was enhanced on the day 1 after the injury, as seen in the cytoplasm of the endothelial cells in the cerebral cortex, hippocampus and cerebellum, and reached the peak level on day 7. From day 14, the expression of enkephalin decreased gradually, but still remained at higher-than-normal level until day 30. The expression of dopamine increased on day 7 and was seen in the cytoplasm of the endothelial cells and in the vascular wall in the cerebral cortex, hippocampus,thalamus and cerebellum, which, however, underwent no notable time-dependent changes.CONCLUSIONS: Cerebral concussion is characterized pathologically by blood circulatory disorder and degeneration and necrosis of the parenchymal cells. Enkephalin and dopamine may participate in the pathophysiological process of cerebral injury after cerebral concussion, and play important roles in blood vessel injury, regulation of blood-brain barrier and the degeneration and necrosis of the neural cells.