CAJ | 학술논문

目的：探讨重组sICAM-1对大鼠白细胞与肺微血管内皮细胞粘附的影响。方法：采用原代培养的大鼠肺微血管内皮细胞及99Tm-HMPAO标记的白细胞，观察不同浓度的sICAM-1、CA7（sICAM-1的单抗）以及sICAM-1*CA7（sICAM-1的二聚体）对PMA刺激的大鼠白细胞与肺微血管内皮细胞粘附的影响。结果：sICAM-1与CA7即使在100 mg/L也不能抑制白细胞与肺微血管内皮细胞的粘附，而其二聚体在20 mg/L和40 mg/L即可抑制42%和50%的细胞粘附（P＜0.05）。结论: sICAM-1对细胞粘附的抑制作用很弱,可能与其单体形式有关。
목적：탐토중조sICAM-1대대서백세포여폐미혈관내피세포점부적영향。방법：채용원대배양적대서폐미혈관내피세포급99Tm-HMPAO표기적백세포，관찰불동농도적sICAM-1、CA7（sICAM-1적단항）이급sICAM-1*CA7（sICAM-1적이취체）대PMA자격적대서백세포여폐미혈관내피세포점부적영향。결과：sICAM-1여CA7즉사재100 mg/L야불능억제백세포여폐미혈관내피세포적점부，이기이취체재20 mg/L화40 mg/L즉가억제42%화50%적세포점부（P＜0.05）。결론: sICAM-1대세포점부적억제작용흔약,가능여기단체형식유관。
AIM:To explore the effect of recombined sICAM-1 on the adhesion of leukocyte to pulmonary microvascular endothelial cell. METHODS:Primary cultured rat pulmonary microvascular endothelial cells were used in this experiment. Leukocyte was labeled with 99　Tm-HMPAO.The effects of different dose of sICAM-1, CA7 and dimeric form of sICAM-1 (sICAM-1*CA7)were tested separately in cell adhesion .RESULTS:sICAM-1 and CA7 could not inhibit cell adhesion even at the concentration of 100 mg/L,while sICAM-1*CA7 at concentrations of 20 mg/L and 40 mg/L could inhibit 42% and 50% of cell adhesion respectively (P＜0.05). CONCLUSION:sICAM-1 has poor inhibitory effect on cell adhesion, probably due to monomeric form.