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万方数据

高等学校化学学报 고등학교화학학보
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES
2001年 4期 563-565 ,共3页

魏泱%丁明玉%李红霞魏泱%丁明玉%李紅霞

위앙%정명옥%리홍하

高效液相色谱法%蒸发光散射检测法%二极管阵列检测法%天麻%天麻甙高效液相色譜法%蒸髮光散射檢測法%二極管陣列檢測法%天痳%天痳甙
고효액상색보법%증발광산사검측법%이겁관진렬검측법%천마%천마대

A normal-phase high performance chromatographic method with evaporative light-scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6∶3∶2) as the mobile phase, was established for the direct determination of active constituents-gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume. An silica gel column Zorbax RX-SIL[46 mm (i.d.)×25 cm, 5 μm] was employed. Regression equations revealed the linear relationships(r=0.998 and 0.999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104.22% and 97.65%, respectively. The contents of GA measured by ELSD and DAD are 13.9 mg/g(2.79% RSD) and 12.1 mg/g(2.99% RSD) respectively in Gastrodia elata Blume. The detection limits of ELSD and DAD(S/N=3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.