植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2001年
3期
326-328
,共3页
魏建华%赵华燕%卢善发%王台%马庆虎%宋艳茹
魏建華%趙華燕%盧善髮%王檯%馬慶虎%宋豔茹
위건화%조화연%로선발%왕태%마경호%송염여
毛白杨%COMT基因%RT-PCR%特异性表达
毛白楊%COMT基因%RT-PCR%特異性錶達
모백양%COMT기인%RT-PCR%특이성표체
The cDNA fragment encoding caffeic acid 3-O-methyltransferase(COMT) in Chinese white poplar (Populus tomentosa Carr.) was isolated and cloned by RT-PCR technique. The size of the cDNA fragment is 1 080 bp, which almost covers the whole cDNA-encoding region. Authors’ cDNA fragment in P. tomentosa shares 98.7% homology with the reported corresponding cDNA in the P. tremuloids at nucleotide level, 99.4% homology at amino acid level, respectively. The analysis of Northern dot hybridization showed that COMT is expressed specifically in the developing secondary xylem of stem during the season of xylem differentiation, which means the linkage between the gene expression for a monolignol biosynthetic enzyme and seasonal regulation of xylem development in woody plant.
